Baculovirus for gene delivery to mammalian cells: Past, present and future

Plasmid
Maysam Mansouri, Philipp Berger

Abstract

Baculovirus is an insect virus which has been used for more than thirty years for production of recombinant proteins in insect cells. However, baculovirus can also be harnessed for efficient gene delivery to mammalian cells if it is equipped with mammalian promoters. This technology is known as BacMam and has been used for gene delivery to immortalized cell lines, stem cells, and primary cells, as well as for gene delivery in animals. Baculovirus has unique features when compared to mammalian viruses. Besides the fact that it is replication-incompetent and does not integrate into the host genome, it has large capacity for foreign DNA. This capacity can for example be used to deliver multiple genes for reprogramming of stem cells, or for delivery of large homology constructs for genome editing. In this review, we provide a brief overview of baculovirus-based gene delivery and its recent applications in therapy and basic research. We also describe how baculovirus is manipulated for efficient transduction in mammalian cells and we highlight possible future improvements.

Citations

May 12, 2020·Journal of Leukocyte Biology·Mario Fragoso-Saavedra, Marco A Vega-López
Jan 1, 2019·Uirusu·Motoko Ikeda
Jul 17, 2020·International Journal of Molecular Sciences·Martin SpillmannPhilipp Berger
Mar 29, 2021·Biotechnology Progress·Mirosław KucharskiEwa Ocłoń
Jul 31, 2021·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Yuanyuan Li, Li-Quan Zhou
Oct 8, 2021·Applied Microbiology and Biotechnology·Alexandra Marisa TargovnikMariano Nicolás Belaich

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