Baculovirus multigene expression vectors and their use for understanding the assembly process of architecturally complex virus particles

Gene
P RoyD H Bishop

Abstract

The baculovirus expression vector is a eukaryotic DNA viral vector for the cloning and expression of foreign genes in cultured lepidopteran insect cells and insects. It has become an important tool for the large-scale production of recombinant proteins for a variety of applications including the structure-function analysis of genes and their gene products. We have developed a number of baculovirus multigene expression vectors and utilized these to understand the assembly process of multicomponent capsid structures of large viruses such as bluetongue virus (BTV), a member of the Orbivirus genus within the family Reoviridae. BTV is some 810 A in diameter and comprised of two protein shells containing four major proteins, VP2, VP5, VP7 and VP3, surrounding a genome of ten double-stranded RNA segments and three minor proteins (VP2, VP4 and VP6). BTV is the etiological agent of a sheep disease that is sometimes fatal in certain parts of the world (e.g., Africa, Asia, and the Americas). Using baculovirus multigene vectors, we have co-expressed various combinations of BTV genes in insect cells and produced structures that mimic the various stages of BTV assembly. For example, co-expressed VP3 and VP7 form BTV core-like particles, whil...Continue Reading

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Citations

Apr 15, 2004·Molecular and Cellular Neurosciences·Raghuram SelvarajuUrsula Boschert
Dec 24, 2002·Life Sciences·Loan N MillerJorge D Brioni
Nov 30, 2004·Nature Biotechnology·Imre BergerTimothy J Richmond
Dec 22, 2015·Virus Research·Sonja MareeJacques Theron
Aug 10, 2010·Protein Expression and Purification·John J KerriganQuinn Lu
Aug 9, 2005·Biochemical and Biophysical Research Communications·Ying-Ju ChenTzong-Yuan Wu
Jun 19, 2012·Bioengineered·Hsin-Yu LuHung-Jen Liu
Feb 25, 2010·Journal of Structural Biology·Simon TrowitzschImre Berger
May 7, 2013·Acta Biochimica Et Biophysica Sinica·Lupeng YeBoxiong Zhong
May 12, 2001·The Journal of Biological Chemistry·A G StephenL H Rome
Jan 9, 2013·Clinical Linguistics & Phonetics·Llorenç AndreuBrian Macwhinney
Oct 1, 2005·International Journal of Language & Communication Disorders·Wendy Best

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