Baculovirus surface display of E(rns) envelope glycoprotein of classical swine fever virus

Journal of Virological Methods
Xin-Gang XuHung-Jen Liu

Abstract

Classical swine fever virus (CSFV) causes significant losses in the pig industry in many countries. E(rns) is an envelope glycoprotein of CSFV which is known to induce virus-neutralizing antibodies and protective immunity in the natural host. In this study, one recombinant baculoviruses BacSC-E(rns) expressing histidine-tagged E(rns) with the transmembrane domain (TM) and cytoplasmic domain (CTD) derived from baculovirus envelope protein gp64 was constructed and its immunizing efficacy was evaluated in a mouse model. After infection, E(rns) was expressed and anchored on the plasma membrane of Sf-9 cells, as demonstrated by Western-blot and confocal microscopy. Immunogold electron microscopy demonstrated that the E(rns) glycoprotein was successfully displayed on the baculoviral envelope. Vaccine tests in animals showed that BacSC-E(rns) elicited significantly higher E(rns) antibody titers in the immunized mouse models than the control group. This demonstrates that the BacSC-E(rns) vaccine can be used potentially against CSFV infections. This is the first report demonstrating the potential of E(rns)-pseudotyped baculovirus as a CSFV vaccine.

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Citations

Aug 3, 2011·Expert Review of Vaccines·Jimmy A Mena, Amine A Kamen
Jun 19, 2012·Bioengineered·Hsin-Yu LuHung-Jen Liu
Aug 27, 2014·Expert Review of Vaccines·Shih-Yeh LinYu-Chen Hu
Jun 21, 2018·Molecular Genetics and Genomics : MGG·Hao ZhengJingchen Sun
Jun 3, 2018·Viruses·Balraj PremanandMookkan Prabakaran

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