BadR (BB0693) controls growth phase-dependent induction of rpoS and bosR in Borrelia burgdorferi via recognizing TAAAATAT motifs

Molecular Microbiology
Zhiming Ouyang, Jianli Zhou

Abstract

In Borrelia burgdorferi (Bb), the alternative sigma factor RpoS plays a central role during Bb's adaptation to ticks and mammals. Previous studies have demonstrated that RpoS is not expressed during the early stages of spirochetal growth or when Bb resides in ticks during the intermolt phase, but the molecular details of these events remain unknown. In the current study, biomagnetic bead separation of rpoS promoter-binding proteins, coupled with genetic inactivation, was employed to identify BadR (BB0693) as a negative regulator that controls growth phase-dependent induction of rpoS and bosR in Bb. When badR was inactivated, the expression of rpoS and bosR was induced only during the early stages of bacterial growth, but not during the stationary growth phase. Recombinant BadR bound to the promoter DNA of rpoS and the regulatory region upstream of bosR via AT-rich TAAAATAT motifs. Mutations in this motif markedly inhibited or abolished rBadR binding. These results suggest that BadR directly influences the expression of both rpoS and bosR in Bb. This newly recognized role for BadR to fine-tune the activation of the RpoN-RpoS pathway at strategic times in Bb's life cycle potentially represents another layer of gene control over σ...Continue Reading

References

Jul 1, 1979·Journal of Bacteriology·D K Shamanna, K E Sanderson
May 1, 1992·Journal of Bacteriology·C SizemoreW Hillen
Mar 31, 1983·The New England Journal of Medicine·A C SteereS E Malawista
Jun 18, 1982·Science·W BurgdorferJ P Davis
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·T G SchwanP A Rosa
May 1, 1993·Journal of Clinical Microbiology·R J PollackA Spielman
Oct 6, 2000·Annual Review of Microbiology·J Stülke, W Hillen
Oct 25, 2001·Proceedings of the National Academy of Sciences of the United States of America·A HübnerM V Norgard
Feb 7, 2002·Proceedings of the National Academy of Sciences of the United States of America·Andrew T RevelMichael V Norgard
Jul 18, 2002·The Journal of Experimental Medicine·Fang Ting LiangErol Fikrig
Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Regine Hengge-Aronis
May 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Joshua R FischerJohn M Leong
Jun 6, 2003·Microbes and Infection·Utpal Pal, Erol Fikrig
Jun 7, 2003·Molecular Microbiology·Janet AlversonD Scott Samuels
Sep 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Xiaofeng F YangMichael V Norgard
Feb 19, 2004·Proceedings of the National Academy of Sciences of the United States of America·Dorothee GrimmPatricia A Rosa
Feb 26, 2004·The Journal of Experimental Medicine·Xiaofeng F YangMichael V Norgard
Sep 18, 2004·Journal of Bacteriology·Laura I KatonaJorge L Benach
Jan 26, 2005·FEMS Microbiology Letters·Kate von Lackum, Brian Stevenson
Feb 18, 2005·Journal of Bacteriology·Matthew Hirsch, Thomas Elliott
Mar 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Mark A FisherFrank C Gherardini
Apr 30, 2005·Proceedings of the National Academy of Sciences of the United States of America·Andrew T RevelMichael V Norgard
Dec 13, 2005·Microbiology and Molecular Biology Reviews : MMBR·Mark J KazmierczakKathryn J Boor
Jun 27, 2006·Molecular Microbiology·Steven J Norris
Dec 13, 2006·Journal of Bacteriology·Alexandra H SmithMichael V Norgard
Dec 13, 2006·Wiener klinische Wochenschrift·Brian StevensonTomasz Bykowski
Jan 16, 2007·Applied and Environmental Microbiology·Jon S BlevinsMichael V Norgard
Jan 30, 2007·Molecular Microbiology·Michael A GilbertD Scott Samuels
Mar 23, 2007·Molecular Microbiology·Qilong XuFang Ting Liang

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Citations

Jun 22, 2016·Infection and Immunity·Zhiming OuyangMichael V Norgard
Jan 20, 2020·Molecular Microbiology·Charlotte MasonZhiming Ouyang
Jul 22, 2018·Frontiers in Cellular and Infection Microbiology·Dan DrecktrahD Scott Samuels
Jul 12, 2020·Nature Reviews. Microbiology·Cheyne KurokawaErol Fikrig
May 6, 2017·Frontiers in Cellular and Infection Microbiology·Xuwu XiangYongliang Lou
Nov 17, 2020·Frontiers in Medicine·Qiang LiuYongliang Lou

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