BAG-1L Protects SH-SY5Y Neuroblastoma Cells Against Hypoxia/Re-oxygenation Through Up-Regulating HSP70 and Activating PI3K/AKT Signaling Pathway

Neurochemical Research
Yun WangYan Qu

Abstract

BCL-2-associated athanogene-1(BAG-1) is a multifunctional and anti-apoptotic protein that was first identified as a binding partner of BCL-2. But the effects and mechanisms for BAG-1 against hypoxic damage is unclear up to now. Whether BAG-1 could protect the human brain against hypoxic damage through up-regulating 70 kDa heat shock proteins (HSP70) and PI3K/AKT pathway activation? In present study, we examined the changes of HSP70 and AKT and p-AKT protein level in SH-SY5Y cells with BAG-1L gene over-expression subjected to hypoxia/re-oxygenation injury. BAG-1L over-expression increased neuronal viability, and it reduced apoptosis of neurons after hypoxia/re-oxygenation for 8 h. BAG-1L over-expression enhanced the HSP70 protein levels and increased p-AKT/total AKT ratio after hypoxia/re-oxygenation for 8 h. These results suggest that BAG-1L over-expression protects against hypoxia/re-oxygenation injury, at least in part, by interacting with HSP70, and by accelerating the activation of PI3K/AKT pathways.

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Citations

Oct 15, 2019·Journal of Cellular Biochemistry·Yan-Kun SongDan Hu
Sep 29, 2020·Bulletin of Experimental Biology and Medicine·I M MadaevaL I Kolesnikova
Jan 29, 2019·British Journal of Neurosurgery·Wen-Hua LiJun Ren
Nov 30, 2018·Journal of Toxicology and Environmental Health. Part a·Yong-Hui ZhangYang-Yang Gao
Feb 1, 2020·Pharmacology & Therapeutics·Elena MariottoSanja Aveic

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Methods Mentioned

BETA
Infection
flow cytometry

Software Mentioned

Rad quantity one
Bio
VILBER LOURMAT
SPSS

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