BAG3 promotes chondrosarcoma progression by upregulating the expression of β-catenin.

Molecular Medicine Reports
Huijuan ShiLiantang Wang

Abstract

To investigate the roles of B‑cell lymphoma‑2 associated athanogene 3 (BAG3) in human chondrosarcoma and the potential mechanisms, the expression levels of BAG3 were detected in the present study, and the associations between BAG3 and clinical pathological parameters, clinical stage as well as the survival of patients were analyzed. The present study detected BAG3 mRNA and protein expression in the normal cartilage cell line HC‑a and in SW1353 chondrosarcoma cells by reverse transcription‑quantitative polymerase chain reaction and western blot analysis. The BAG3 protein expression in 59 cases of chondrosarcoma, 30 patients with endogenous chondroma and 8 cases of normal cartilage was semi-quantitatively analyzed using the immunohistochemical method. In addition, the BAG3 protein expression level, the clinical pathological parameters, clinical stage and the survival time of patients with chondrosarcoma were analyzed. The plasmid transfection method was employed to upregulate the expression BAG3 and small RNA interference to downregulate the expression of BAG3 in SW1353 cells. The expression levels of BAG3 protein and mRNA were significantly increased in the chondrosarcoma cell line when compared with the normal cartilage cell li...Continue Reading

References

Mar 24, 2005·Journal of Cellular Biochemistry·Toshihisa Komori
Oct 23, 2009·The Biochemical Journal·Margit FuchsJacques Landry
Jun 11, 2010·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Stefania StaibanoEmilio Morelli
Apr 8, 2011·Cell Death & Disease·A RosatiM C Turco
Jul 4, 2013·The American Journal of Gastroenterology·Antonia FalcoMaria Caterina Turco
Oct 2, 2013·Biochimica Et Biophysica Acta·De-Hui KongHua-Qin Wang
Oct 9, 2013·Annals of Oncology : Official Journal of the European Society for Medical Oncology·A ItalianoJ Y Blay
Jan 1, 2012·Central European Journal of Urology·Andrzej NowickiJoanna Duda-Szymańska
Jul 6, 2014·Cancer Research·Teresa A ColvinMichael Y Sherman
Jan 8, 2015·Molecular Cancer Therapeutics·Xiaokai LiJason E Gestwicki
Nov 19, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Huiyong ShiZhongfa Xu
Mar 11, 2016·Journal of Oncology Practice·Michael J WagnerRobert S Benjamin
Apr 29, 2016·International Journal of Oncology·Shutaro HabataTsuyoshi Saito

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Citations

Dec 12, 2019·Anti-cancer Agents in Medicinal Chemistry·Roghayeh IjabiAmirhossein Sahebkar

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Methods Mentioned

BETA
Antibody Sampler
transfections
transfection

Software Mentioned

Statistical Package for the Social Sciences ( SPSS )

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