Bagasse hydrolyzates from Agave tequilana as substrates for succinic acid production by Actinobacillus succinogenes in batch and repeated batch reactor

Bioresource Technology
Rosa Isela Corona-GonzálezGuillermo Toriz

Abstract

The aim of this work was to obtain fermentable sugars by enzymatic or acid hydrolyses of Agave tequilana Weber bagasse in order to produce succinic acid with Actinobacillus succinogenes. Hydrolyses were carried out with mineral acids (sulfuric and hydrochloric acids) or a commercial cellulolytic enzyme, and were optimized statistically by a response surface methodology, having as factors the concentration of acid/enzyme and time of hydrolysis. The concentration of sugars obtained at optimal conditions for each hydrolysis were 21.7, 22.4y 19.8g/L for H2SO4, HCl and the enzymatic preparation respectively. Concerning succinic acid production, the enzymatic hydrolyzates resulted in the highest yield (0.446g/g) and productivity (0.57g/Lh) using A. succinogenes in a batch reactor system. Repeated batch fermentation with immobilized A. succinogenes in agar and with the enzymatic hydrolyzates resulted in a maximum concentration of succinic acid of 33.6g/L from 87.2g/L monosaccharides after 5 cycles in 40h, obtaining a productivity of 1.32g/Lh.

References

Feb 24, 1999·International Journal of Systematic Bacteriology·M V GuettlerM K Jain
Oct 3, 2008·Applied Biochemistry and Biotechnology·Kequan ChenHao Wu
Oct 1, 2010·Journal of Industrial Microbiology & Biotechnology·Elcio Ribeiro Borges, Nei Pereira
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Nov 8, 2012·Bioresource Technology·Jose A Perez-PimientaBlake A Simmons
Nov 29, 2012·Bioresource Technology·Min JiangPing Wei
May 23, 2014·Bioresource Technology·Rosa Isela Corona-GonzálezCarlos Pelayo-Ortiz

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Citations

Apr 27, 2017·3 Biotech·Miguel A Medina-MoralesCristóbal N Aguilar
Sep 28, 2018·Applied Microbiology and Biotechnology·Wubliker DessieMin Jiang
Jun 7, 2019·Journal of Environmental Management·Bernd WeberIván G Martínez-Cienfuegos
Dec 23, 2021·Preparative Biochemistry & Biotechnology·Nurul Adela BukhariJamaliah Md Jahim

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