Baicalein attenuates the quorum sensing-controlled virulence factors of Pseudomonas aeruginosa and relieves the inflammatory response in P. aeruginosa-infected macrophages by downregulating the MAPK and NFκB signal-transduction pathways

Drug Design, Development and Therapy
Jing LuoYi-Qiang Chen

Abstract

Burgeoning antibiotic resistance and unfavorable outcomes of inflammatory injury after Pseudomonas aeruginosa infection have necessitated the development of novel agents that not only target quorum sensing (QS) but also combat inflammatory injury with the least risk of resistance. This study aimed to assess the anti-QS and anti-inflammatory activities of baicalein, a traditional herbal medicine that is widely used in the People's Republic of China, against P. aeruginosa infection. We found that subminimum inhibitory concentrations of baicalein efficiently interfered with the QS-signaling pathway of P. aeruginosa via downregulation of the transcription of QS-regulated genes and the translation of QS-signaling molecules. This interference resulted in the global attenuation of QS-controlled virulence factors, such as motility and biofilm formation, and the secretion into the culture supernatant of extracellular virulence factors, including pyocyanin, LasA protease, LasB elastase, and rhamnolipids. Moreover, we examined the anti-inflammatory activity of baicalein and its mode of action via a P. aeruginosa-infected macrophage model to address its therapeutic effect. Baicalein reduced the P. aeruginosa-induced secretion of the inflam...Continue Reading

Citations

Oct 4, 2017·Folia Microbiologica·Irena KolouchováJan Masák
Oct 23, 2018·Phytotherapy Research : PTR·Faegheh FarhadiMilad Iranshahy
Nov 7, 2019·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Moumita MajumdarDijendra Nath Roy
Nov 17, 2016·Proceedings of the National Academy of Sciences of the United States of America·Nina M Høyland-KroghsboBonnie L Bassler
Feb 23, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Tanveer AhmedV Venkateswara Sarma
Aug 17, 2020·Microorganisms·Waleska Stephanie da Cruz NizerJoerg Overhage
Nov 4, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Francesca GuzzoBrigida D'Abrosca
Jan 23, 2018·Journal of Agricultural and Food Chemistry·Jin-Wei ZhouAi-Qun Jia

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Methods Mentioned

BETA
scanning electron microscopy
PCR
enzyme-linked immunosorbent assay
ELISA
Protein Assay
nuclear translocation

Software Mentioned

SPSS
GraphPad
GraphPad Prism

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