Baicalin exerts protective effects against lipopolysaccharide-induced acute lung injury by regulating the crosstalk between the CX3CL1-CX3CR1 axis and NF-κB pathway in CX3CL1-knockout mice

International Journal of Molecular Medicine
Xin-Min DingQin-Zhi Xu

Abstract

Acute lung injury (ALI) as a serious diseases with high mortality and is considered a threat to human health and life. A number of studies have focused on the treatment and prevention of lung injury. However, the molecular mechanisms responsible for the development of lung injury are not yet fully understood, and this has impeded the development of effective drugs and treatment strategies. Hence, in the present study, mice with lipopolysaccharide (LPS)‑induced ALI were used as a model to investigate the crosstalk between the CX3CL1-CX3CR1 axis and the nuclear factor (NF)-κB signaling pathway in the process of lung injury. CX3CL1-knockout (CX3CL1-KO or CX3CL1-/-) mice were used to examine the role of the CX3CL1-CX3CR1 axis in LPS-induced lung injury. We used baicalin, a natural product, to investigate its anti-inflammatory effects and its protective effects against lung injury. Western blot analysis, reverse transcription-quantitavie PCR (RT-qPCR), immunohistochemistry, enzyme-linked immunosorbent assay (ELISA) and the analysis of biochemical indicators were used to determine the key signaling pathway involved in the development of lung injury. The results indicated that, on the one hand, baicalin exerted potent anti-inflammator...Continue Reading

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Sep 19, 2016·Acta Neuropathologica Communications·Sabine SellnerThomas Blank
May 19, 2018·Journal of Cellular Biochemistry·Mahesh M Gouda, Yashodhar P Bhandary
Sep 14, 2017·Journal of Cellular Biochemistry·Mahesh M GoudaYashodhar P Bhandary
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Jan 10, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zihe DingZunpeng Shu
Apr 1, 2021·Phytotherapy Research : PTR·Abhishek GourUtpal Nandi

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Methods Mentioned

BETA
ELISA
bronchoalveolar lavage
PCR
protein assay
X-ray
electrophoresis

Software Mentioned

GraphPad PRISM
GraphPad
DeCyder

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