Baicalin improves IVM of pig oocytes and subsequent preimplantation embryo development by inhibiting apoptosis

Reproduction, Fertility, and Development
Qing GuoJin-Dan Kang

Abstract

Baicalin, a monomer of flavonoids extracted from dried roots of Scutellaria baicalensis, is used to treat female infertility. However, the effect of baicalin on oocyte maturation is unknown. In this study we investigated the effects of baicalin on the IVM of pig oocytes and subsequent embryo development following parthenogenetic activation (PA). We found that 0.1µgmL-1 baicalin significantly (P<0.05) increased the IVM rate of oocytes compared with the non-treatment (control) group by reducing levels of reactive oxygen species (ROS). In addition, the mRNA expression of genes related to nuclear maturation and cumulus cell expansion, mitochondrial membrane potential and ATP content was significantly (P<0.05) higher in baicalin-treated than control oocytes. To determine whether baicalin treatment during IVM of pig oocytes improves subsequent development of PA embryos, we measured the cleavage and blastocyst formation rates, as well as the number of cells per blastocyst. All these parameters were significantly (P<0.05) higher in the baicalin-treated than control group. In conclusion, this study demonstrates that baicalin improves pig oocyte maturation and subsequent embryo development invitro by inhibiting production of ROS and redu...Continue Reading

References

Apr 3, 2001·Nature Reviews. Genetics·T Hassold, P Hunt
Apr 12, 2002·Animal Reproduction Science·Ming Yuan Yang, Rajadurai Rajamahendran
Jan 18, 2003·Reproduction : the Official Journal of the Society for the Study of Fertility·Rafael A FissoreJeremy Smyth
May 7, 2005·Free Radical Research·Shruti AgarwalNilanjan Roy
Sep 22, 2006·Methods in Molecular Biology·James Buck WilliamsMark E Westhusin
Oct 13, 2006·Bioscience, Biotechnology, and Biochemistry·Wen-Hsin HuangChing-Huey Yang
Jan 16, 2007·Current Topics in Developmental Biology·A J HarveyC A Brenner
Feb 3, 2007·Zygote : the Biology of Gametes and Early Embryos·Masashi NaganoYoshiyuki Takahashi
Feb 29, 2008·The American Journal of Chinese Medicine·Xiu-Hui ZhongXiao-Dan Wang
Dec 20, 2008·Stem Cells·Cesar A SommerGustavo Mostoslavsky
Feb 18, 2009·The American Journal of Chinese Medicine·Ai-Tuan MaLi-Gen Meng
Mar 14, 2009·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Xiao Ying TianMin Xu
Jan 19, 2010·Annual Review of Pathology·Douglas C WallaceVincent Procaccio
Mar 24, 2011·Reproduction, Fertility, and Development·Hisataka IwataYashunori Monji
Sep 20, 2011·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Yi-Fei WenSatendra Kumar Nirala
Jan 5, 2013·Current Topics in Developmental Biology·Janet E HoltKeith T Jones
Aug 28, 2013·Proceedings of the National Academy of Sciences of the United States of America·Karen WigglesworthJohn J Eppig
Sep 1, 2016·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Huifen WangShiyu Du
Jan 18, 2017·Journal of Pineal Research·Jun-Xue JinByeong Chun Lee
Dec 29, 2017·Animal Science Journal = Nihon Chikusan Gakkaihō·Meihong ShiQingwang Li

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Citations

Aug 6, 2020·In Vitro Cellular & Developmental Biology. Animal·Emma HicksBrian D Whitaker
Jan 15, 2021·Zygote : the Biology of Gametes and Early Embryos·Sam Zarbakhsh
Jun 15, 2021·Journal of Cellular Physiology·Ling WangFenge Li
Aug 22, 2020·Theriogenology·Eui Hyun KimByeong Chun Lee

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis