Baicalin inhibits Escherichia coli isolates in bovine mastitic milk and reduces antimicrobial resistance

Journal of Dairy Science
Q Y ZhaoW M Zhang

Abstract

In this study, we aimed to evaluate the inhibitory effect of baicalin on Escherichia coli in vitro and the effects of baicalin treatment on antimicrobial resistance of the E. coli isolates. Through isolation, purification, and identification, a total of 56 E. coli strains were isolated from 341 mastitic milk samples. The study of inhibition effect of baicalin on the E. coli strains in vitro was focused on permeability and morphology of the isolates using an alkaline phosphatase kit and scanning electron microscopy. Furthermore, the resistance spectrum of the isolates to the common antimicrobial agents was tested at sub-minimum inhibitory concentrations of baicalin by the agar dilution method. Extended-spectrum β-lactamase and plasmid-mediated quinolone resistance genes were amplified by PCR before and after incubation with baicalin. The results revealed that baicalin has certain inhibitory effects on the isolates in vitro. The alkaline phosphatase enzyme activity was significantly increased from 1.246 to 2.377 U/100 mL, and the surface of E. coli was concave and shriveled. Analysis of the resistance spectrum and PCR amplification showed that, after administration with baicalin, the sensitivity of most strains to the selected an...Continue Reading

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Citations

Dec 21, 2018·International Journal of Environmental Health Research·Mohamad Fawzi MahomoodallyMuhammad Zakariyyah Aumeeruddy
May 17, 2019·International Journal of Molecular Sciences·Shulin FuChien-An Andy Hu
Oct 8, 2019·Current Cancer Drug Targets·Martyna Godzieba, Slawomir Ciesielski
Aug 12, 2020·Asian-Australasian Journal of Animal Sciences·Wei Nee Cheng, Sung Gu Han
May 1, 2021·Animals : an Open Access Journal From MDPI·Baishuang YinXuezhao Sun
Jul 6, 2021·European Journal of Medicinal Chemistry·Ting WangJianyou Shi

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