PMID: 10686591Feb 25, 2000

Balance of two secretion pathways of nerve growth factor in PC12 cells changes during the progression of their differentiation, with a decrease in constitutive secretion in more differentiated cells

Journal of Neuroscience Research
Hiroshi NomotoS Furukawa

Abstract

Proteins are secreted from animal cells by either a constitutive or a regulated pathway. When cDNA of nerve growth factor (NGF) was introduced into PC12 cells, these cells produced and secreted active NGF, where NGF was secreted not only in constitutive but also in activity-dependent regulated way according to the results of pulse-chase and ELISA studies. The regulated secretion was caused by depolarization, cyclic AMP analogue, or beta-adrenergic agonist but not by glutamate or carbachol. Because these transfected cells differentiated into a morphology indistinguishable from that incubated with NGF protein, we next compared the secretion pathways of NGF from PC12 cells at different stages of the differentiation. NGF was secreted in both constitutive and regulated way at 2 and 7 days after the transfection of NGF-cDNA, but the constitutive secretion of NGF from the more differentiated cells of Day 7 was decreased and mature NGF tended to accumulate in the cells. These results indicate that the neurotrophin secretion mechanism is intimately regulated in the course of the differentiation of PC12 cells. Such a change in the protein secretion pathway might have an profound role in the development of neurons.

References

Feb 16, 2013·Biochemical and Biophysical Research Communications·Hiroshi NomotoShoei Furukawa
Mar 31, 2007·Biochemical and Biophysical Research Communications·Hiroshi NomotoShoei Furukawa
Jan 9, 2007·Biochemical and Biophysical Research Communications·Akihiro MouriShoei Furukawa
Sep 4, 2015·International Journal of Urology : Official Journal of the Japanese Urological Association·Yi-Hao LinFirouz Daneshgari
Dec 1, 2005·Biomaterials·Kevin J Whittlesey, Lonnie D Shea
Apr 26, 2006·Bioscience, Biotechnology, and Biochemistry·Noriko HattoriShoei Furukawa
Sep 5, 2002·Journal of Neuroscience Research·Hisanori ItoShoei Furukawa

Citations

Jan 1, 1990·Annual Review of Physiology·A M Brown, L Birnbaumer
Jan 1, 1990·Developmental Neuroscience·M P ShortX O Breakefield
Jan 1, 1990·Progress in Growth Factor Research·Y A Barde
Mar 1, 1983·Journal of Neurochemistry·S FurukawaK Hayashi
Oct 27, 1995·Science·H Thoenen
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·E S LevineM R Plummer
Feb 1, 1993·Trends in Biochemical Sciences·R A BradshawJ Murray-Rust
Oct 11, 1996·The Journal of Biological Chemistry·J V HeymachE M Shooter
Jan 1, 1996·Critical Reviews in Neurobiology·J Wess
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·M CanossaH Thoenen
Mar 26, 1998·Journal of Neuroscience Research·J C MöllerE M Shooter
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·A KrüttgenE M Shooter

Related Concepts

Biochemical Pathway
Nerve Growth Factor gamma Subunit
Transfection
Secretory Pathway
Science of Morphology
Proteins, Recombinant DNA
Neurons
Nerve Growth Factor
COS-7 Cells
Analog

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.