Band-broadening suppressed effect in long turned geometry channel and high-sensitive analysis of DNA sample by using floating electrokinetic supercharging on a microchip.

Biomicrofluidics
Zhongqi XuTakeshi Hirokawa

Abstract

A featured microchip owning three big reservoirs and long turned geometry channel was designed to improve the detection limit of DNA fragments by using floating electrokinetic supercharging (FEKS) method. The novel design matches the FEKS preconcentration needs of a large sample volume introduction with electrokinetic injection (EKI), as well as long duration of isotachophoresis (ITP) process to enrich low concentration sample. In the curved channel [ approximately 45.6 mm long between port 1 (P1) and the intersection point of two channels], EKI and ITP were performed while the side port 3 (P3) was electrically floated. The turn-induced band broadening with or without ITP process was investigated by a computer simulation (using CFD-ACE+ software) when the analytes traveling through the U-shaped geometry. It was found that the channel curvature determined the extent of band broadening, however, which could be effectively eliminated by the way of ITP. After the ITP-stacked zones passed the intersection point from P1, they were rapidly destacked for separation and detection from ITP to zone electrophoresis by using leading ions from P3. The FEKS carried on the novel chip successfully contributed to higher sensitivities of DNA frag...Continue Reading

References

Jul 2, 2002·Analytical Chemistry·Pierre-Alain AurouxAndreas Manz
Jul 27, 2002·Analytical Chemistry·Stewart K Griffiths, Robert H Nilson
Jun 15, 2004·Analytical Chemistry·Torsten VilknerAndreas Manz
Dec 7, 2007·Electrophoresis·Maximilien BlasJean-Louis Rocca
Aug 12, 2008·Journal of Separation Science·Kenji SueyoshiKoji Otsuka
Jan 1, 2008·Biomicrofluidics·Liyu LiuPing Sheng
Jan 1, 2009·Biomicrofluidics·Christopher ChurchXiangchun Xuan

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Citations

Feb 11, 2012·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Fumihiko KitagawaKoji Otsuka
Aug 23, 2011·Journal of Chromatography. a·Michelle M MeighanMichael C Breadmore
Jul 28, 2011·Journal of Separation Science·Mohamed Dawod, Doo Soo Chung
Dec 21, 2010·Electrophoresis·Petr GebauerPetr Boček
Feb 4, 2011·Electrophoresis·Stacy M KenyonMark A Hayes
Jul 10, 2013·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Fumitoshi HirokawaKazuhisa Uchiyama
Jul 22, 2021·Electrophoresis·Wolfgang Thormann, Richard A Mosher

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Methods Mentioned

BETA
electrophoresis
chip
chips

Software Mentioned

ITP
CFD
ACE +

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