Bank vole monoclonal antibodies against Puumala virus envelope glycoproteins: identification of epitopes involved in neutralization

Archives of Virology
A Lundkvist, B Niklasson

Abstract

Bank vole (Clethrionomys glareolus) monoclonal antibodies (MAbs) against the two envelope glycoproteins (G 1 and G 2) of the Puumala (PUU) virus were generated and characterized. Analyses of the MAbs' antigen and epitope specificities showed non-overlapping reactivities of one anti-G 1 and two anti-G 2 MAbs. A significant neutralizing activity was shown by the anti-G 1 and one of the anti-G 2 MAbs, suggesting the existence of at least one neutralizing domain on each of the two glycoproteins. The two neutralizing MAbs reacted with eight PUU-related (serotype 3) virus strains, but did not react with Hantaan, Seoul, or Prospect Hill viruses in an immunofluorescence assay, indicating reactivity with epitopes unique for PUU virus. The non-neutralizing anti-G 2 MAb also reacted with Seoul virus, revealing the presence of a conserved G 2-epitope common for PUU and Seoul viruses, not involved in neutralization. Competitive binding of the MAbs and sera from nephropathia epidemica patients indicated that the defined neutralizing and non-neutralizing epitopes of the glycoproteins were immunodominant also in humans. In another experiment, magnetic beads coated with two MAbs were bound with the virus glycoproteins and used for selective enr...Continue Reading

References

Mar 1, 1978·The Journal of Infectious Diseases·H W LeeK M Johnson
Dec 1, 1991·The American Journal of Tropical Medicine and Hygiene·B NiklassonE Tkachenko
Mar 1, 1991·The Journal of General Virology·S L RuoJ B McCormick
Jan 1, 1991·Archives of Virology·S L RuoS P Fisher-Hoch
Mar 1, 1990·The Journal of General Virology·R M Elliott
May 1, 1989·The Journal of General Virology·A SanchezM P Kiley
Jun 21, 1989·Journal of Immunological Methods·F A OssendorpM De Boer
Oct 1, 1988·The Journal of General Virology·H SheshberadaranE A Tkachenko
Dec 1, 1988·The Journal of General Virology·L J BaekD C Gajdusek
Feb 1, 1980·The Journal of Infectious Diseases·M Brummer-KorvenkontioJ Lähdevirta
Nov 1, 1982·The Journal of Infectious Diseases·H W LeeK M Johnson
May 5, 1984·Lancet·B Niklasson, J Le Duc
Jan 1, 1980·Journal of Cancer Research and Clinical Oncology·N H Nielsen, J P Hansen

❮ Previous
Next ❯

Citations

Feb 24, 2000·Journal of Medical Virology·C de Carvalho NicacioE Björling
Jan 1, 1995·Archives of Virology·T DzagurovaA Lundkvist
Jul 26, 2012·Archives of Virology·Mathias SchlegelBernd Köllner
Jul 17, 2012·Virus Genes·Ivan C KuroltAlemka Markotić
Sep 1, 1992·Journal of Virological Methods·J HörlingB Niklasson
May 25, 2005·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Jonas SchmidtRainer Ulrich
Mar 31, 2004·Microbes and Infection·Masanori TerajimaFrancis A Ennis
Nov 16, 2001·Microbes and Infection·D H KrügerA Lundkvist A
Sep 5, 2002·The Journal of Experimental Medicine·Heather L Van EppsFrancis A Ennis
Apr 16, 2002·Viral Immunology·A LundkvistR Ulrich
Jan 7, 2003·Viral Immunology·Svetlana F Khaiboullina, Stephen C St Jeor
Mar 27, 2003·Vector Borne and Zoonotic Diseases·Tarja SironenAlexander Plyusnin
Jan 27, 2005·Viral Immunology·Piet MaesMarc Van Ranst
Dec 15, 2012·Clinical and Vaccine Immunology : CVI·R L BrocatoJ W Hooper
Oct 16, 2009·Journal of Virology·Jussi HepojokiHilkka Lankinen
Aug 31, 2006·Journal of Virology·Agne AlminaiteAlexander Plyusnin
Jun 12, 2009·Journal of Virology·Pamela R HallRichard S Larson
Nov 2, 2001·Journal of Virology·T SironenA Plyusnin
Jan 1, 1995·Critical Reviews in Clinical Laboratory Sciences·B HjelleA A Scott
May 17, 2000·The Journal of General Virology·C de Carvalho NicacioA Lundkvist

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Epigenetics Insights from Twin Studies

Find the latest research on epigenetics and twin studies here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.