bantam microRNA is a negative regulator of the Drosophila decapentaplegic pathway

Fly
Nanci S KaneYing Li

Abstract

Decapentaplegic (Dpp), the Drosophila homolog of the vertebrate bone morphogenetic protein (BMP2/4), is crucial for patterning and growth in many developmental contexts. The Dpp pathway is regulated at many different levels to exquisitely control its activity. We show that bantam (ban), a microRNA, modulates Dpp signaling activity. Over expression of ban decreases phosphorylated Mothers against decapentaplegic (Mad) levels and negatively affects Dpp pathway transcriptional target genes, while null mutant clones of ban upregulate the pathway. We provide evidence that dpp upregulates ban in the wing imaginal disc, and attenuation of Dpp signaling results in a reduction of ban expression, showing that they function in a feedback loop. Furthermore, we show that this feedback loop is important for maintaining anterior-posterior compartment boundary stability in the wing disc through regulation of optomotor blind (omb), a known target of the pathway. Our results support a model that ban functions with dpp in a negative feedback loop.

References

Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·R W PadgettW M Gelbart
Jun 3, 1998·Methods : a Companion to Methods in Enzymology·C B Phelps, A H Brand
Jun 8, 1999·Developmental Biology·L A Raftery, D J Sutherland
Jun 25, 1999·Cell Death and Differentiation·V Van de BorJ Silber
Dec 12, 2003·Development·Damali N Martin, Eric H Baehrecke
Dec 20, 2003·Nature·Robert J Johnston, Oliver Hobert
Feb 26, 2004·Progress in Molecular and Subcellular Biology·L ParkerK Arora
Apr 6, 2004·Developmental Biology·David del Alamo RodríguezFernando J Díaz-Benjumea
Sep 17, 2004·Development·Francisco A MartínGinés Morata
Oct 27, 2004·PLoS Biology·Bino JohnDebora S Marks
Feb 22, 2005·PLoS Biology·Julius BrenneckeStephen M Cohen
Mar 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Harlan RobinsRichard W Padgett
Apr 5, 2005·Nature Genetics·Azra KrekNikolaus Rajewsky
Mar 1, 2006·Current Opinion in Genetics & Development·Richard W Carthew
Aug 22, 2006·Nature Structural & Molecular Biology·Dominic Didiano, Oliver Hobert
Sep 5, 2006·Current Biology : CB·Riitta NoloGeorg Halder
Sep 19, 2006·Nature Genetics·Eunjoo ChoKenneth D Irvine
Oct 5, 2006·Molecular Cell·Julide BilenNancy M Bonini
May 18, 2007·Annual Review of Cell and Developmental Biology·Natascha Bushati, Stephen M Cohen
Aug 19, 2007·Nature Reviews. Genetics·Markus Affolter, Konrad Basler
Dec 14, 2007·Development·Nir YakobyStanislav Y Shvartsman
Jan 4, 2008·Mechanisms of Development·Jie ShenGert O Pflugfelder

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Citations

Jan 10, 2020·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Jia GouHans G Othmer
Oct 8, 2020·Genetics·Michael J TexadaKim Rewitz
Dec 1, 2020·Cellular Signalling·Priyanka SamjiKarunagaran Devarajan
May 7, 2021·Genetics·Michael J TexadaKim Rewitz

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Methods Mentioned

BETA
immunoprecipitation
confocal microscopy

Software Mentioned

miRanda
pMad
TargetScan

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