Bantam regulates the axonal geometry of Drosophila larval brain by modulating actin regulator enabled

Invertebrate Neuroscience : in
Animesh Banerjee, Jagat Kumar Roy

Abstract

During development, axonogenesis, an integral part of neurogenesis, is based on well-concerted events comprising generation, rearrangement, migration, elongation, and adhesion of neurons. Actin, specifically the crosstalk between the guardians of actin polymerization, like enabled, chickadee, capping protein plays an essential role in crafting several events of axonogenesis. Recent evidences reflect multifaceted role of microRNA during axonogenesis. Here, we investigated the role of bantam miRNA, a well-established miRNA in Drosophila, in regulating the actin organization during brain development. Our immunofluorescence studies showed altered arrangement of neurons and actin filaments whereas both qPCR and western blot revealed elevated expression of enabled, one of the actin modulators in bantam mutant background. Collectively, our results clearly demonstrate that bantam plays an instrumental role in shaping the axon architecture regulating the actin geometry through its modulator enabled.

References

Nov 15, 1996·Science·M Tessier-Lavigne, C S Goodman
Sep 15, 2004·Trends in Biochemical Sciences·Martin A Wear, John A Cooper
Jun 9, 2005·The Journal of Biological Chemistry·Melanie BarzikDorothy A Schafer
Nov 23, 2006·Nature Reviews. Neuroscience·Kenneth S Kosik
Nov 18, 2008·Current Biology : CB·Daniela BerdnikLiqun Luo
Nov 26, 2010·Cold Spring Harbor Perspectives in Biology·Erik W DentFrank B Gertler
May 10, 2011·Cell Stem Cell·Ralph A NeumüllerJuergen A Knoblich
Jul 18, 2012·Genes & Development·Homaira NawabiZhigang He
Nov 8, 2012·Development·Catarina C F Homem, Juergen A Knoblich
Jan 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Arthur LuhurNicholas S Sokol
Aug 20, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Cecilia S LuDavid Van Vactor
Dec 9, 2014·Trends in Cell Biology·Joana A Vidigal, Andrea Ventura
Dec 5, 2015·Frontiers in Neurology·Gregory M DavisRoger Pocock

❮ Previous
Next ❯

Methods Mentioned

BETA
reverse transcription PCR

Software Mentioned

Adobe Photoshop7
Sigma Plot
LSM 510 Meta
Adobe Photoshop

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Adhesion Molecules in the Brain

Cell adhesion molecules found on cell surface help cells bind with other cells or the extracellular matrix to maintain structure and function. Here is the latest research on their role in the brain.