Baohuoside-1, a novel immunosuppressive molecule, inhibits lymphocyte activation in vitro and in vivo

Transplantation
Anlun MaHuifang Chen

Abstract

We evaluated the in vitro and in vivo immunosuppressive effects of baohuoside-1 (B1), a novel flavonoid isolated from Epimedium davidii. Proliferation assay was used to determine the antiproliferative properties on T-cell and B-cell proliferation. Flow cytometry analysis was applied to detect changes of phenotypes on activated cells. B1 inhibits the lymphocyte proliferation activated by polyclonal mitogens and mixed lymphocyte reaction with a 50% inhibitory concentration of low micromolar concentration. Also, B1 suppressed T-cell activation in T cell receptor/CD3-mediated signaling pathways in a dose- and time-dependent manner. The suppression of B1 was not simply a result of a toxic effect and was recovered by withdrawing the drug. B1 down-regulated the expression of some phenotype molecules. In Ca(2+)-independent or -dependent antigen stimulation, although B1 had different inhibitive patterns on CD69 expression stimulated by phorbol 12-myristate 13-acetate (PMA) or Ca2+ ionophore, it inhibited T-cell proliferation induced by CD3/CD28 or PMA/ionomycin and partially blocked that induced by PMA/CD28. Interestingly, an additive inhibition between B1 and tacrolimus (FK506) was found in the CD69 expression stimulated by PMA/CD28 an...Continue Reading

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Citations

Jan 11, 2011·Journal of Pharmaceutical and Biomedical Analysis·S P LiB Yang
Oct 5, 2007·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Li-Kuan PeiPei-Gen Xiao
Aug 6, 2015·The American Journal of Chinese Medicine·Xiao-Jia ChenYi-Tao Wang
Oct 15, 2005·Transplant Immunology·Anlun MaHuifang Chen

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