DOI: 10.1101/478743Nov 27, 2018Paper

BAPC-assisted CRISPR/Cas9 System: Targeted Delivery into Adult Ovaries for Heritable Germline Gene Editing (Arthropoda: Hemiptera)

BioRxiv : the Preprint Server for Biology
Wayne B. HunterJohn Tomich

Abstract

Innovative technologies aimed at reducing psyllid populations using targeting RNA suppression, like RNAi, or gene-editing tools, like CRISPR/Cas9 have the potential to reduce psyllid vectors and disrupt pathogen transmission. Breakthroughs that improve gene editing in psyllids, such as BAPC-assisted-CRISPR/Cas9 System, enabled delivery by injection of CRISPR/Cas9 components directly into nymphs and adult females. Injection near ovaries produced heritable germline gene editing in subsequent generations, G0, G1, G2. This method opens the world of gene editing across arthropods and bypasses the need for microinjection of eggs. Effective development of therapeutic treatments against psyllid and leafhopper vectors, providing strategies for maintaining sustainable citrus and grapevine industries.

Related Concepts

Arthropods
Birth
Citrus plant
Disease Vectors
Genetic Vectors
Germ Line
Hemiptera
Microinjections
Ovary
Ovum

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