Bar-HRM for authenticating soursop (Annona muricata) tea

Scientific Reports
Maslin Osathanunkul

Abstract

Drinking soursop (Annona muricata) tea has become popular in Thailand due to recent findings about the medicinal properties of soursop tea regarding anti-cancer in particular. Consequently, numerous A. muricata tea products were found to be sold on markets and relatively expensive. It is almost impossible to identify the plant species component in the tea bag or powder products using traditional methods which are based on morphological characters. Therefore, a main objective of this study is to develop a molecular method called Bar-HRM (DNA barcoding coupled with High Resolution Melting) for authenticating A. muricata products. Three chloroplast regions including matK, rbcL and trnL were selected for in silico analyses. The findings show that rbcL is the most suitable region to be used for species identification in HRM analysis. Eleven A. muricata herbal products were purchased and tested with rbcL primers. Results from melting profile indicated that three out of eleven tested products were adulterated with other Annona species. It is believed that the Annona products are adulterated to increase the quantity and to make more profit. Notably, all of the tested products purchased from local producers were found to contain herbal ...Continue Reading

References

Mar 17, 2001·Environmental Health Perspectives·D S Fabricant, N R Farnsworth
Jul 29, 2004·Complementary Therapies in Nursing & Midwifery·Roxanne StruthersBeverly Patchell
Dec 16, 2006·Nucleic Acids Research·Pierre TaberletEske Willerslev
Jun 4, 2011·PloS One·Peter M HollingsworthDamon P Little
Sep 4, 2012·Asian Pacific Journal of Cancer Prevention : APJCP·Sulaiman HamizahC I Tan
Oct 15, 2013·BMC Medicine·Steven G NewmasterSubramanyam Ragupathy
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Dec 18, 2013·Scientific Reports·Damon P Little, Marc L Jeanson
Nov 7, 2014·Methods in Molecular Biology·Natasha de VereCharlotte Long
Dec 17, 2014·Genome Génome / Conseil National De Recherches Canada·Damon P Little
Jul 18, 2015·International Journal of Molecular Sciences·Soheil Zorofchian MoghadamtousiHabsah Abdul Kadir
Aug 19, 2015·Frontiers in Plant Science·Nerea Larranaga, José I Hormaza
Oct 16, 2015·Phytotherapy Research : PTR·Hossein Hosseinzadeh, Marjan Nassiri-Asl
Mar 2, 2016·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Maslin OsathanunkulHugo de Boer
Mar 29, 2018·BMC Complementary and Alternative Medicine·Maslin OsathanunkulPanagiotis Madesis

❮ Previous
Next ❯

Citations

Jan 5, 2020·Journal of the Science of Food and Agriculture·Yanfei DingCheng Zhu
Dec 18, 2020·Comprehensive Reviews in Food Science and Food Safety·Liliana GrazinaIsabel Mafra

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

uMeltSM
MEGA
uMelt SM application

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.