BarA-UvrY two-component system regulates virulence of uropathogenic E. coli CFT073.

PloS One
Senthilkumar PalaniyandiSuman Mukhopadhyay

Abstract

Uropathogenic Escherichia coli (UPEC), a member of extraintestinal pathogenic E. coli, cause ∼80% of community-acquired urinary tract infections (UTI) in humans. UPEC initiates its colonization in epithelial cells lining the urinary tract with a complicated life cycle, replicating and persisting in intracellular and extracellular niches. Consequently, UPEC causes cystitis and more severe form of pyelonephritis. To further understand the virulence characteristics of UPEC, we investigated the roles of BarA-UvrY two-component system (TCS) in regulating UPEC virulence. Our results showed that mutation of BarA-UvrY TCS significantly decreased the virulence of UPEC CFT073, as assessed by mouse urinary tract infection, chicken embryo killing assay, and cytotoxicity assay on human kidney and uroepithelial cell lines. Furthermore, mutation of either barA or uvrY gene reduced the production of hemolysin, lipopolysaccharide (LPS), proinflammatory cytokines (TNF-α and IL-6) and chemokine (IL-8). The virulence phenotype was restored similar to that of wild-type by complementation of either barA or uvrY gene in trans. In addition, we discussed a possible link between the BarA-UvrY TCS and CsrA in positively and negatively controlling virulen...Continue Reading

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Citations

Aug 25, 2015·Biochimica Et Biophysica Acta·Laura C Ristow, Rodney A Welch
Aug 21, 2013·Veterinary Microbiology·Senthilkumar PalaniyandiSuman Mukhopadhyay
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Methods Mentioned

BETA
Electrophoresis
PCR

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