BARD1 expression during spermatogenesis is associated with apoptosis and hormonally regulated

Biology of Reproduction
Anis FekiIrmgard Irminger-Finger

Abstract

The BRCA1-binding RING-finger domain protein BARD1 may act conjointly with BRCA1 in DNA repair and in ubiquitination, but it may also induce apoptosis in a BRCA1-independent manner. In this study, we have investigated BARD1 expression during spermatogenesis. In contrast with BRCA1, which is expressed only in meiotic spermatocytes and early round spermatids, BARD1 is expressed during all stages of spermatogenesis. However, while spermatogonia expressed full-length BARD1 mRNA, later stages of spermatocyte precursors express predominantly a novel, shorter splice form BARD1beta. BARD1beta lacks the BRCA1-interacting RING finger but maintains its proapoptotic activity. Consistently, BRCA1 can counteract the proapoptotic activity of full-length BARD1 but not of BARD1beta. Several lines of evidence suggest that BARD1 is involved in proapoptotic signaling in testis: i) both BARD1 isoforms are mostly found in cells that stain positive for TUNEL, Bax, and activated caspase 3; ii) BARD1beta, capable of inducing apoptosis even in the presence of BRCA1, is specifically expressed in BRCA1-positive later stages of spermatogenesis; iii) antiapoptotic hormonal stimulation leads to BARD1 downregulation; and iv) BARD1 expression is associated wit...Continue Reading

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Citations

Jan 2, 2007·Apoptosis : an International Journal on Programmed Cell Death·Carlos LizamaRicardo D Moreno
Apr 25, 2006·Nature Reviews. Cancer·Irmgard Irminger-Finger, Charles Edward Jefford
Mar 19, 2005·Genes & Development·Allison E FalenderJoAnne S Richards
Jun 23, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Judith C SpornBarbara Jung
Jan 8, 2016·The International Journal of Biochemistry & Cell Biology·Irmgard Irminger-FingerMaxim Pilyugin
Feb 12, 2005·Reproductive Biomedicine Online·Ingrid Ehrmann, David J Elliott
Jun 9, 2007·The International Journal of Biochemistry & Cell Biology·Lin LiIrmgard Irminger-Finger
Mar 24, 2009·Birth Defects Research. Part C, Embryo Today : Reviews·Tin-Lap LeeWai-Yee Chan
Sep 10, 2005·International Journal of Cancer. Journal International Du Cancer·Jian-Yu WuIrmgard Irminger-Finger
Aug 5, 2011·International Journal of Cancer. Journal International Du Cancer·Yong-Qiang ZhangIrmgard Irminger-Finger
Sep 30, 2015·Respiratory Research·Pierre-Alain AndréIrmgard Irminger-Finger
Apr 28, 2016·Molecular Carcinogenesis·Farhadul IslamAlfred King-Yin Lam
Jan 3, 2018·Genes·Flora CimminoMario Capasso
Jan 16, 2009·Biology of Reproduction·Anne ForandJacqueline Bernardino-Sgherri
Feb 4, 2021·Genes·Anna JasiakEwa Iżycka-Świeszewska

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Methods Mentioned

BETA
PCR
flow cytometry
transfection

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