Barium- and calcium-permeable channels open at negative membrane potentials in rat ventricular myocytes.

The Journal of Membrane Biology
A CoulombeE Coraboeuf

Abstract

Ca2+- and Ba2+-permeable channel activity from adult rat ventricular myocytes, spontaneously appeared in the three single-channel recording configurations: cell-attached, and excised inside-out or outside-out membrane patches. Single-channel activity was recorded at steady-state applied membrane potentials including the entire range of physiologic values, and displayed no "rundown" in excised patches. This activity occurred in irregular bursts separated by quiescent periods of 5 to 20 min in cell-attached membrane patches, whereas in excised patch experiments, this period was reduced to 2 to 10 min. During activity, a variety of kinetic behaviors could be observed with more or less complex gating patterns. Three conductance levels: 22, 45 and 78 pS were routinely observed in the same excised membrane patch, sometimes combining to give a larger level. These channels were significantly permeable to divalent cations and showed little or no permeability to potassium or sodium ions. The inorganic blockers of voltage-gated Ca channels, cobalt (2 mM), cadmium (0.5 mM) or nickel (3 mM), had no apparent effect on these spontaneous unitary currents carried by barium ions. Under 10(5) M Bay K 8644 or nitrendipine, the activity was clearly...Continue Reading

References

Jan 1, 1986·Biomedica Biochimica Acta·B NiliusR W Tsien
May 1, 1986·Pflügers Archiv : European journal of physiology·T F McDonaldD Pelzer
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R Mitra, M Morad
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B MartinacC Kung
Jan 1, 1987·The Journal of Membrane Biology·M D CoyneI B Levitan
Nov 1, 1987·Biophysical Journal·J A TalvenheimoM T Nelson
Mar 1, 1988·Biophysical Journal·J Ma, R Coronado
Sep 1, 1988·Pflügers Archiv : European journal of physiology·M T KirberJ J Singer
Jan 18, 1989·Biochimica Et Biophysica Acta·H Meves, K Nagy
Jan 10, 1985·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·D DiFrancesco, D Noble
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Sep 1, 1983·Pflügers Archiv : European journal of physiology·A CavaliéW Trautwein
Jul 1, 1963·The Journal of Physiology·R NIEDERGERKE

❮ Previous
Next ❯

Citations

Jan 29, 2000·Pflügers Archiv : European journal of physiology·J Mould, A F Dulhunty
Oct 24, 1998·Progress in Biophysics and Molecular Biology·E Carmeliet, K Mubagwa
Dec 1, 1991·The Journal of Cell Biology·P R TurnerR A Steinhardt
Feb 7, 2001·The Journal of General Physiology·C R WeberD M Bers
May 19, 2009·Canadian Journal of Physiology and Pharmacology·Meghan R MontTimothy P Geisbuhler
Jun 27, 2007·The American Journal of Pathology·Larissa LipskaiaAnne-Marie Lompré
Jul 26, 2005·Pharmacology & Therapeutics·Penelope A Boyden, Henk ter Keurs
Sep 3, 1998·Biophysical Journal·C A Obejero-PazA Scarpa
Aug 13, 2004·Journal of Neurobiology·Andrew K Tryba, Jan-Marino Ramirez
Oct 14, 2011·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Jae Boum YoumJin Han
Apr 13, 2007·Physiological Reviews·Henk E D J Ter Keurs, Penelope A Boyden
May 27, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Julio AlvarezGuy Vassort
Feb 5, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Joëlle Abi-CharStéphane N Hatem
Apr 5, 2001·American Journal of Physiology. Cell Physiology·L ValenciaP Poujeol
Oct 1, 1996·Pflügers Archiv : European journal of physiology·C Lamont, D A Eisner
Oct 11, 1996·The American Journal of Physiology·F W HopfR A Steinhardt
Dec 1, 1994·The American Journal of Physiology·J S FrankB S Tuana
Jul 1, 1994·The American Journal of Physiology·H MatsunagaP A Friedman
Aug 14, 1998·Neurobiology of Disease·C G Carlson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.