Barium, TEA and sodium sensitive potassium channels are present in the human placental syncytiotrophoblast apical membrane

Placenta
P DíazG Riquelme

Abstract

The human placental syncytiotrophoblast (hSTB) is a polarized epithelial structure, without paracellular routes, forming the main barrier for materno-fetal exchange. There is ample evidence suggesting the presence of potassium (K(+)) channels in the placental apical membrane; which could contribute to membrane potential and volume regulation. We have therefore examined the K(+) currents of isolated apical membranes from human term placenta using electrophysiological methods: reconstitution of ion channels from apical membranes into giant liposomes (single channel recordings, patch clamp method) or their functional transplantation into Xenopus laevis oocytes (total currents recording, voltage clamp method). Single channel recording experiments show the presence of K(+) channels in the hSTB microvillous membrane sensitive to Tetraethylammonium (TEA) and Barium (Ba(+2)). Patch current activity was diminished 50% and 70% by 20 mmol/L TEA and 5 mmol/L Ba(+2) respectively. The more frequent conductance was approximately 73pS, however several levels of current were detected suggesting the presence of more than one type of K(+) channel. In addition, sodium (Na(+)) sensitivity was detected in the patch current thus, over 10 mmol/L Na(+)...Continue Reading

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Citations

Sep 23, 2008·The Journal of Membrane Biology·Valeria Godoy, Gloria Riquelme
May 17, 2011·The Journal of Membrane Biology·Gloria RiquelmeCarolina Rodríguez
Sep 5, 2012·Journal of Obstetrics and Gynaecology : the Journal of the Institute of Obstetrics and Gynaecology·G K FyfeM Wareing
Oct 14, 2018·The Journal of Biological Chemistry·Evgeniy PotapenkoRoberto Docampo

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