Barley metallothioneins: MT3 and MT4 are localized in the grain aleurone layer and show differential zinc binding.

Plant Physiology
Josefine N HegelundJan K Schjoerring

Abstract

Metallothioneins (MTs) are low-molecular-weight, cysteine-rich proteins believed to play a role in cytosolic zinc (Zn) and copper (Cu) homeostasis. However, evidence for the functional properties of MTs has been hampered by methodological problems in the isolation and characterization of the proteins. Here, we document that barley (Hordeum vulgare) MT3 and MT4 proteins exist in planta and that they differ in tissue localization as well as in metal coordination chemistry. Combined transcriptional and histological analyses showed temporal and spatial correlations between transcript levels and protein abundance during grain development. MT3 was present in tissues of both maternal and filial origin throughout grain filling. In contrast, MT4 was confined to the embryo and aleurone layer, where it appeared during tissue specialization and remained until maturity. Using state-of-the-art speciation analysis by size-exclusion chromatography inductively coupled plasma mass spectrometry and electrospray ionization time-of-flight mass spectrometry on recombinant MT3 and MT4, their specificity and capacity for metal ion binding were quantified, showing a strong preferential Zn binding relative to Cu and cadmium (Cd) in MT4, which was not th...Continue Reading

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Citations

Jun 22, 2014·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Mireia TomasSílvia Atrian
Jul 3, 2013·Plant, Cell & Environment·Michaela SchillerJan K Schjoerring
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May 3, 2014·Plant Physiology·Shuanglong HuangClaudio Stasolla
Apr 30, 2021·Journal of Plant Physiology·Houqing ZengYiyong Zhu

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