Barley stripe mosaic virus γb Protein Subverts Autophagy to Promote Viral Infection by Disrupting the ATG7-ATG8 Interaction

The Plant Cell
Meng YangDawei Li

Abstract

Autophagy is a conserved defense strategy against viral infection. However, little is known about the counterdefense strategies of plant viruses involving interference with autophagy. Here, we show that γb protein from Barley stripe mosaic virus (BSMV), a positive single-stranded RNA virus, directly interacts with AUTOPHAGY PROTEIN7 (ATG7). BSMV infection suppresses autophagy, and overexpression of γb protein is sufficient to inhibit autophagy. Furthermore, silencing of autophagy-related gene ATG5 and ATG7 in Nicotiana benthamiana plants enhanced BSMV accumulation and viral symptoms, indicating that autophagy plays an antiviral role in BSMV infection. Molecular analyses indicated that γb interferes with the interaction of ATG7 with ATG8 in a competitive manner, whereas a single point mutation in γb, Tyr29Ala (Y29A), made this protein deficient in the interaction with ATG7, which was correlated with the abolishment of autophagy inhibition. Consistently, the mutant BSMVY29A virus showed reduced symptom severity and viral accumulation. Taken together, our findings reveal that BSMV γb protein subverts autophagy-mediated antiviral defense by disrupting the ATG7-ATG8 interaction to promote plant RNA virus infection, and they provide ...Continue Reading

Citations

Nov 16, 2019·Molecular Plant-microbe Interactions : MPMI·Qian Chen, Taiyun Wei
Apr 24, 2020·Journal of Integrative Plant Biology·Hua QiShi Xiao
Jun 13, 2020·Annual Review of Virology·Meng YangYule Liu
Mar 19, 2019·Journal of Experimental Botany·Aayushi ShuklaAnders Hafrén
Sep 4, 2020·International Journal of Molecular Sciences·Jie RanJian-Zhong Liu
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Aug 10, 2019·Molecular Plant Pathology·Nirbhay Kumar KushwahaDaniel Hofius
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