Barley stripe mosaic virus (BSMV) as a virus-induced gene silencing vector in maize seedlings

Virus Genes
Sridhar JarugulaLucy R Stewart

Abstract

Barley stripe mosaic virus (BSMV) was the first reported and still widely used virus-induced gene silencing (VIGS) vector for monocotyledons including wheat and barley. Despite BSMV's reported infectivity on maize (Zea mays), the use of the virus as a vector in maize has not been optimized. Here, we assayed infectivity of BSMV in different maize cultivars by vascular puncture inoculation. Through knockdown of the endogenous host phytoene desaturase gene, we demonstrate for the first time that BSMV can be used as a VIGS vector in maize. This adds BSMV to the repertoire of tools available for functional studies in maize.

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Citations

Mar 10, 2019·Plant Molecular Biology·Ravi Kant, Indranil Dasgupta
Aug 13, 2020·Plant Direct·Sizolwenkosi MlotshwaLucy R Stewart
Aug 15, 2019·Plant Direct·Yu MeiSteven A Whitham
Oct 8, 2020·Plant Methods·Irene N GentzelGuo-Liang Wang
Nov 2, 2021·Frontiers in Plant Science·Juliana Erika de Carvalho Teixeira YassitepePaulo Arruda

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