Barriers-on-chips: Measurement of barrier function of tissues in organs-on-chips

Biomicrofluidics
Yusuf B ArıkAndries D van der Meer

Abstract

Disruption of tissue barriers formed by cells is an integral part of the pathophysiology of many diseases. Therefore, a thorough understanding of tissue barrier function is essential when studying the causes and mechanisms of disease as well as when developing novel treatments. In vitro methods play an integral role in understanding tissue barrier function, and several techniques have been developed in order to evaluate barrier integrity of cultured cell layers, from microscopy imaging of cell-cell adhesion proteins to measuring ionic currents, to flux of water or transport of molecules across cellular barriers. Unfortunately, many of the current in vitro methods suffer from not fully recapitulating the microenvironment of tissues and organs. Recently, organ-on-chip devices have emerged to overcome this challenge. Organs-on-chips are microfluidic cell culture devices with continuously perfused microchannels inhabited by living cells. Freedom of changing the design of device architecture offers the opportunity of recapitulating the in vivo physiological environment while measuring barrier function. Assessment of barriers in organs-on-chips can be challenging as they may require dedicated setups and have smaller volumes that are ...Continue Reading

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Citations

Sep 25, 2019·Biosensors·Sebastian Rudi Adam KratzMario Rothbauer
Feb 27, 2020·Advanced Healthcare Materials·Shantanu PradhanJohn H Slater
Aug 7, 2020·Lab on a Chip·M ZakharovaL I Segerink
Jul 11, 2020·Advanced Biosystems·Arbel Artzy-SchnirmanJosué Sznitman
Jul 16, 2020·Materials·Liubov BakhchovaUlrike Steinmann
May 6, 2019·Bioengineering·Kimia Kamal, Ben Waldau
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Aug 1, 2021·Drug Discovery Today·Nicole Kleinstreuer, Anthony Holmes
Sep 1, 2021·Critical Reviews in Toxicology·Marlon R SchneiderRob J Vandebriel

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Methods Mentioned

BETA
in
chip
fluorescence microscopy
chips

Software Mentioned

TEER

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