Basal Body Protein TbSAF1 Is Required for Microtubule Quartet Anchorage to the Basal Bodies in Trypanosoma brucei.

MBio
Xiaoduo DongCynthia Y He

Abstract

Sperm flagellar protein 1 (Spef1, also known as CLAMP) is a microtubule-associated protein involved in various microtubule-related functions from ciliary motility to polarized cell movement and planar cell polarity. In Trypanosoma brucei, the causative agent of trypanosomiasis, a single Spef1 ortholog (TbSpef1) is associated with a microtubule quartet (MtQ), which is in close association with several single-copied organelles and is required for their coordinated biogenesis during the cell cycle. Here, we investigated the interaction network of TbSpef1 using BioID, a proximity-dependent protein-protein interaction screening method. Characterization of selected candidates provided a molecular description of TbSpef1-MtQ interactions with nearby cytoskeletal structures. Of particular interest, we identified a new basal body protein TbSAF1, which is required for TbSpef1-MtQ anchorage to the basal bodies. The results demonstrate that MtQ-basal body anchorage is critical for the spatial organization of cytoskeletal organelles, as well as the morphology of the membrane-bound flagellar pocket where endocytosis takes place in this parasite.IMPORTANCE Trypanosoma brucei contains a large array of single-copied organelles and structures. Th...Continue Reading

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Citations

Dec 15, 2020·Trends in Parasitology·Clare HallidayJack D Sunter
Oct 28, 2021·Microbiology Spectrum·Christine E Broster ReixDerrick R Robinson

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Methods Mentioned

BETA
fluorescence microscopy
transmission electron microscopy
electron tomography
fluorescence
flow cytometry
transfections

Software Mentioned

BioID
Adobe Photoshop
Adobe Illustrator
ImageJ
IMOD
CytExpert
MASCOT Server

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