Basal lamina and other extracellular matrix produced by bovine granulosa cells in anchorage-independent culture

Cell and Tissue Research
H F RodgersRaymond J Rodgers

Abstract

Bovine granulosa cells from 3-7 mm follicles were cultured without anchorage in soft agar/methylcellulose solution for 14 days, with or without 50 ng/ml basic fibroblast growth factor. The granulosa cells divided to form colonies of cells. These were analysed by light and electron microscopy, immunohistochemistry and Western immunoblotting. In approximately 20% of the colonies extracellular matrix was clearly visible at the light-microscope level. Ultrastructurally the matrix resembled a basal lamina 30-100 nm thick and was composed of tangled fibres or cords. Unidentified spherical structures of less than 50 nm diameter were sometimes present and attached to this basal lamina. The basal lamina of follicles had similar features, except that the basal lamina produced in vitro was a large aggregate of many convoluted layers. The cells produced collagen type IV and the cellular form of fibronectin. Intercellular areas not associated with basal lamina were identified. Ruthenium red staining revealed these areas to be rich in proteoglycan granules. Free granules were clustered near the cell surface, and the lumina of these areas were rich in fibres decorated with ruthenium red. This material did not resemble follicular fluid of antr...Continue Reading

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Citations

Dec 9, 1997·Fertility and Sterility·G V EriksenN Uldbjerg
Aug 30, 2000·Molecular and Cellular Endocrinology·R J RodgersI L van Wezel
Feb 13, 2001·Molecular and Cellular Endocrinology·R J RodgersT C Lavranos
Jun 5, 2002·Molecular and Cellular Endocrinology·R J Rodgers, H F Irving Rodgers
Jul 20, 1999·Molecular and Cellular Endocrinology·R J RodgersH F Irving-Rodgers
Sep 1, 2006·Seminars in Reproductive Medicine·Courtney B BerkholtzTeresa K Woodruff
Mar 14, 2013·BioMed Research International·Katarzyna Kossowska-Tomaszczuk, Christian De Geyter
Jul 20, 2006·In Vitro Cellular & Developmental Biology. Animal·Michel A Horisberger
Feb 22, 2012·Molecular Aspects of Medicine·Dan ZhangHe-Feng Huang
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May 15, 2007·Micron : the International Research and Review Journal for Microscopy·Wolf Dietrich Krautgartner, Ljubomir Vitkov
Aug 7, 2004·Matrix Biology : Journal of the International Society for Matrix Biology·Helen F Irving-RodgersRaymond J Rodgers
Feb 21, 2007·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Samir SahaSib S Roy
Jul 9, 2010·Biology of Reproduction·David T Armstrong, Raymond J Rodgers
Nov 26, 1998·Biology of Reproduction·H F RodgersR J Rodgers
Feb 19, 2010·Biology of Reproduction·Raymond J Rodgers, Helen F Irving-Rodgers
Dec 30, 2014·Endocrine Reviews·Katja HummitzschRaymond J Rodgers

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