Basal medium development for serum-free culture: a historical perspective.

Cytotechnology
D JaymeT Shimada

Abstract

The evolution of basal synthetic formulations to support mammalian cell culture applications has been facilitated by the contributions of many investigators. Definition of minimally-required nutrient categories by Harry Eagle in the 1950's spawned an iterative process of continuous modification and refinement of the exogenous environment to cultivate new cell types and to support emerging applications of cultured mammalian cells. Key historical elements are traced, leading to the development of high potency, basal nutrient formulations capable of sustaining serum-free proliferation and biological production. Emerging techniques for alimentation of fed batch and continuous perfusion bioreactors, using partial nutrient concentrates deduced from spent medium analysis, can enhance medium utilization and bioreactor productivity.

References

Aug 1, 1991·Bio/technology·D W Jayme, D J Greenwold
Oct 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·H MurakamiH Omura
Feb 20, 1967·JAMA : the Journal of the American Medical Association·G E MooreH A Franklin
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H MurakamiT Kano-Sueoka
Dec 1, 1980·Cell·D Barnes, G Sato
Jan 1, 1994·Biotechnology Progress·T A BibilaJ G Aunins
Jul 1, 1959·Virology·R DULBECCO, G FREEMAN
Feb 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·R G HAM

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Citations

Mar 3, 2005·Biotechnology Letters·Bok-Hwan ChunNamhyun Chung
Aug 13, 2008·Applied Biochemistry and Biotechnology·Suthasinee NilsangSudip Kumar Rakshit
Mar 31, 2015·Journal of Extracellular Vesicles·Erez EitanMark P Mattson
Aug 20, 2019·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Max T BuchkoJayan Nagendran
Apr 1, 1999·Biotechnology and Bioengineering·J CôtéA Kamen
Nov 13, 2015·Biotechnology Progress·Laurie B HazeltineAnli Ouyang
Apr 14, 2015·Biotechnology Progress·David BrühlmannHervé Broly
Sep 25, 2017·Biotechnology Letters·Nicola McGillicuddyJonathan Bones
May 13, 2020·Biotechnology Progress·Andjela BajicStephen D Waldman
Jun 19, 2021·Biotechnology and Bioengineering·Timothy BrantleySarwat Khattak
Apr 7, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J van der ValkG Gstraunthaler

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