Basal membrane heparan sulphate proteoglycan expression during wound healing in human skin

The Journal of Pathology
M P AndriessenJ Schalkwijk

Abstract

Heparan sulphate proteoglycans (HSPGs) are integral components of the basement membrane (BM) in various tissues. HSPGs are important in the assembly and structure of the BM, and their putative functions include regulation of basement membrane permeability, binding of growth factors, and a role in cellular adhesion. In this study the expression of HSPG was examined during wound healing in human skin, using monoclonal antibodies (MAbs) that recognize the HSPG core protein and two different heparan sulphate (HS) epitopes, and the dynamics of HSPG expression were investigated in relation to epidermal cellular proliferation and permeability of the BM. Healing of excisional wounds in healthy volunteers was studied from day 0 up to 1 year. Intact human skin showed strong continuous staining of the dermo-epidermal BM and the vascular BM with all MAbs. Up to day 4 after wounding, staining for HSPG was absent under the ingrowing epidermis, with any of the MAbs, indicating that no complete BM was present. From day 7 onwards, the BM of the neo-epidermis showed positive staining for the HSPG core protein and a low sulphated HS epitope, and after day 14, the staining intensity was similar to normal skin. The staining patterns of these HSPG e...Continue Reading

References

Jan 1, 1992·Progress in Growth Factor Research·P MartinJ McCluskey
Jan 1, 1992·Annual Review of Cell Biology·M BernfieldE J Lose
Jan 1, 1992·Critical Reviews in Clinical Laboratory Sciences·D M Templeton
Jan 1, 1991·The British Journal of Dermatology·J SchalkwijkE J Mackie
Mar 8, 1991·Cell·E Ruoslahti, Y Yamaguchi
Nov 1, 1990·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P BiancoP G Robey
Dec 1, 1988·The Journal of Cell Biology·E J MackieD Liverani
Feb 1, 1988·Archives of Dermatology·R A Clark
Jan 1, 1988·Annual Review of Cell Biology·E Ruoslahti
Aug 1, 1981·The Journal of Investigative Dermatology·J R StanleyS I Katz
Jul 1, 1981·Kidney International·L G HunsickerS J Shaffer
Feb 1, 1995·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·O OksalaH Larjava
Feb 17, 1995·The Journal of Biological Chemistry·G TsenG J Cole
Jan 1, 1993·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·C P KiritsyS E Lynch
May 1, 1993·Biochemical Society Transactions·J E Turnbull, J T Gallagher
Dec 29, 1995·The Journal of Biological Chemistry·J van den BornU Lindahl
Jan 1, 1996·The Journal of Pathology·M A LatijnhouwersJ Schalkwijk

❮ Previous
Next ❯

Citations

Oct 23, 2009·The Journal of General Virology·Tatevik R BroutianNeil D Christensen
Apr 17, 2004·The Journal of Investigative Dermatology·Toon F C M SmetsersToin H van Kuppevelt
Jan 30, 1999·The Journal of Pathology·M P AndriessenJ Schalkwijk
Jan 26, 2006·Journal of Dental Research·A A DeCarlo, J M Whitelock
Apr 30, 2003·Journal of Neurobiology·Min Jung KimGregory J Cole
Jan 10, 1998·The Journal of Pathology·J A McGrath, R A Eady
Jul 31, 2004·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·C Scully, J-V Bagan
Jan 29, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Eyal ZchariaRinat Abramovitch
Jul 14, 2005·Chemical Reviews·John M Whitelock, Renato V Iozzo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.