Basal steroidogenic activity of adrenocortical cells is increased 10-fold by coculture with chromaffin cells

Endocrinology
A HaidanM Ehrhart-Bornstein

Abstract

Historically, catecholamine-producing chromaffin cells and steroid-producing adrenocortical cells have been regarded as two independent endocrine systems that are united under a common capsule to form the adrenal gland. There is increasing evidence for bidirectional interactions, with regulatory influences of adrenocortical secretory products on adrenomedullary functions and vice versa. However, the direct involvement of chromaffin cells on the regulation and maintenance of cortical function has not yet been demonstrated. Therefore, we analyzed glucocorticoid secretion and P450 messenger RNA (mRNA) expression in bovine adrenocortical cells in cocultures with chromaffin cells compared with those in pure cortical cell cultures. Cortisol release from cortical cells in coculture with chromaffin cells was 10 times as high (mean +/- SEM, 1035 +/- 119%) as that from the same number of isolated cortical cells (100 +/- 11%). By a [3H]thymidine incorporation assay, it was demonstrated that this effect was not due to a higher proliferation rate. Northern analysis revealed an increasing expression of P450(17alpha) mRNA in the coculture from days 1-5, whereas in isolated cortical cells, P450(17alpha) mRNA decreased, leading to a 6-fold diff...Continue Reading

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Citations

May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Salvatore AlesciMonika Ehrhart-Bornstein
Nov 14, 2003·Proceedings of the National Academy of Sciences of the United States of America·M Ehrhart-BornsteinS R Bornstein
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·S R BornsteinS Roffler-Tarlov
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Apr 18, 2012·Cell Transplantation·Vladimir VukicevicMonika Ehrhart-Bornstein
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Apr 3, 2001·European Journal of Clinical Investigation·G PäthS R Bornstein
Sep 28, 2017·Hypertension·Teresa M SecciaGian Paolo Rossi
May 25, 2006·Neuroendocrinology·Holger S WillenbergStefan R Bornstein
Jul 18, 2009·Stem Cells·Kuei-Fang ChungMonika Ehrhart-Bornstein
Nov 17, 2010·Cellular and Molecular Neurobiology·Monika Ehrhart-BornsteinStefan R Bornstein
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Jul 17, 2004·Endocrine Practice : Official Journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists·Syed Abbas RazaRobert D Blank

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