PMID: 2015227Apr 16, 1991Paper

Base- and sequence-dependent binding of aristololactam beta-D-glucoside to deoxyribonucleic acid

Biochemistry
R NandiM Maiti

Abstract

The dependence on base-pair composition and sequence specificity of the (aristololactam beta-D-glucoside)-DNA interaction was examined by spectrophotometric, spectrofluorometric, spectropolarimetric, thermal melting, thermodynamic, and viscometric studies. Binding of this alkaloid to various natural and synthetic DNAs was dependent upon the base composition and sequences of DNA. The binding parameters obtained from spectrophotometric analysis, according to an excluded-site model, indicated a relatively high affinity of the alkaloid binding to GC-rich DNA and alternating GC polymer. This affinity was further evidenced by the quenching of fluorescence intensity, decrease in quantum yield, and perturbations in circular dichroic spectrum. The alkaloid stabilized all DNAs against thermal denaturation. The temperature dependence of the binding constants was used to estimate the thermodynamic parameters involved in the complex formation of the alkaloid with various DNAs. The negative enthalpy and entropy change increased with increasing GC content of DNA and also compensated one another to produce a relatively small Gibbs free energy change. Viscometric studies showed that in the strong binding region the increase of contour length of...Continue Reading

References

Jul 30, 1990·Biochimica Et Biophysica Acta·R NandiM Maiti
Jan 1, 1990·Journal of Natural Products·J M CassadyC J Chang
Sep 1, 1989·Equine Veterinary Journal·P M McCue, W D Wilson
Dec 15, 1987·Biochemistry·J B ChairesM J Waring
Apr 1, 1987·The Biochemical Journal·S NeidleJ V Skelly
Aug 1, 1987·Journal of Biomolecular Structure & Dynamics·M Maiti, R Nandi
Jul 1, 1987·Analytical Biochemistry·M Maiti, R Nandi
Feb 1, 1985·Biochemical Pharmacology·R Nandi, M Maiti
Nov 1, 1985·Photochemistry and Photobiology·R NandiM Maiti
May 26, 1972·Biochemical and Biophysical Research Communications·J RamsteinM Leng
Dec 20, 1974·Nature·M J Waring, L P Wakelin
Apr 28, 1970·Journal of Molecular Biology·R D Wells, J E Larson
Oct 1, 1968·Biopolymers·D M Crothers
Jan 1, 1981·Annual Review of Biochemistry·M J Waring
Jun 7, 1982·FEBS Letters·M MaitiK Chaudhuri
Jun 9, 1981·Biochemistry·J L Bresloff, D M Crothers

❮ Previous
Next ❯

Citations

Nov 2, 1993·Biochemical Pharmacology·G S KumarM Maiti
Nov 29, 1994·Biochemical Pharmacology·A Sen, M Maiti
Nov 8, 2003·Bioorganic & Medicinal Chemistry·Gopinatha Suresh KumarMotilal Maiti
Jun 11, 2005·Bioorganic & Medicinal Chemistry·Kakali BhadraMotilal Maiti
Aug 1, 1991·Journal of Biomolecular Structure & Dynamics·D DebnathM Maiti
Nov 20, 2002·Journal of Biomolecular Structure & Dynamics·Motilal MaitiRuma Nandi
Jul 12, 2003·Journal of Biomolecular Structure & Dynamics·Arghya RayMotilal Maiti
Jun 30, 2007·Biochimica Et Biophysica Acta·Prabal Giri, Gopinatha Suresh Kumar
May 9, 2006·International Journal of Biological Macromolecules·Prabal GiriGopinatha Suresh Kumar
Jun 13, 2015·Journal of Biomolecular Structure & Dynamics·Abhi Das, Gopinatha Suresh Kumar

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.