PMID: 8604296Feb 15, 1996Paper

Base and sugar requirements for RNA cleavage of essential nucleoside residues in internal loop B of the hairpin ribozyme: implications for secondary structure

Nucleic Acids Research
S SchmidtM J Gait

Abstract

The hairpin ribozyme is a small self-cleaving RNA that can be engineered for RNA cleavage in trans and has potential as a therapeutic agent. We have used a chemical synthesis approach to study the requirements of hairpin RNA cleavage for sugar and base moieties in residues of internal loop B, an essential region in one of the two ribozyme domains. Individual nucleosides were substituted by either a 2'-deoxy-nucleoside, an abasic residue, or a C3-spacer (propyl linker) and the abilities of the modified ribozymes to cleave an RNA substrate were studied in comparison with the wild-type ribozyme. From these results, together with previous studies, we propose a new model for the potential secondary structure of internal loop B of the hairpin ribozyme.

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Citations

Feb 17, 2001·Biopolymers·David M. J. Lilley
Jul 17, 1998·Current Opinion in Chemical Biology·N G Walter, J M Burke
May 23, 2002·RNA·Timothy J Wilson, David M J Lilley
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Nov 3, 2016·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Yoshiaki Kitamura
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Aug 7, 2014·Chemical Communications : Chem Comm·Katarzyna Wawrzyniak-Turek, Claudia Höbartner
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Oct 13, 2006·Biophysical Chemistry·D R DuckettD M Lilley
Jun 8, 2021·Nucleic Acids Research·Christina E WeinbergZasha Weinberg

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