PMID: 9421514Feb 28, 1998Paper

Base complementarity in helix 2 of the central pseudoknot in 16S rRNA is essential for ribosome functioning

Nucleic Acids Research
R A PootJ van Duin

Abstract

Helix 2 of the central pseudoknot structure in Escherichia coli 16S rRNA is formed by a long-distance interaction between nt 17-19 and 918-916, resulting in three base pairs: U17-A918, C18-G917and A19-U916. Previous work has shown that disruption of the central base pair abolishes ribosomal activity. We have mutated the first and last base pairs and tested the mutants for their translational activity in vivo , using a specialized ribosome system. Mutations that disrupt Watson-Crick base pairing result in strongly impaired translational activity. An exception is the mutation U916-->G, creating an A.G pair, which shows almost no decrease in activity. Mutations that maintain base complementarity have little or no impact on translational efficiency. Some of the introduced base pair substitutions substantially alter the stability of helix 2, but this does not influence ribosome functioning, neither at 42 nor at 28 degrees C. Therefore, our results do not support models in which the pseudoknot is periodically disrupted. Rather, the central pseudoknot structure is suggested to function as a permanent structural element necessary for proper organization in the center of the 30S subunit.

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Citations

Apr 4, 2009·Nature Structural & Molecular Biology·Priya Ramaswamy, Sarah A Woodson
Feb 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Biswajoy Roy-ChaudhuriGloria M Culver
Jun 5, 2008·Nucleic Acids Research·Lon M Chubiz, Christopher V Rao
Feb 16, 2016·Scientific Reports·Burak GulenLoren Dean Williams
Sep 22, 2001·Structure·G M Culver
Jun 15, 1999·Journal of Molecular Biology·L Holmberg, H F Noller
Jan 20, 2004·Nature Structural & Molecular Biology·Kristi L Holmes, Gloria M Culver
Sep 10, 2014·Nature Structural & Molecular Biology·Neha Gupta, Gloria M Culver
Jul 18, 2008·Biochemical Society Transactions·Ian BrierleySimon Pennell
Mar 16, 2017·Frontiers in Bioengineering and Biotechnology·Kentaro MiyazakiMiyuki Tsukuda

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