Base-pairing of 23 S rRNA ends is essential for ribosomal large subunit assembly

Journal of Molecular Biology
Aivar Liiv, Jaanus Remme

Abstract

In ribosomal RNA precursors the spacer sequences bracketing mature 16 S and 23 S rRNA are base-paired to form long helices (processing stems). In pre-23 S rRNA, the processing stem is continued by eight base-pairs of mature 23 S rRNA known as helix 1. Recently, we have found that any part of 23 S rRNA between positions 40 and 2773 could be deleted without the loss of ribosome-like particle formation, while both end regions were indispensable. In this paper we have analyzed the role of the 5' and 3' end regions of 23 S rRNA during ribosomal 50 S assembly in vivo by using mutants of the 23 S rRNA gene. Deletions and substitutions in both strands of the helix 1 lead to the loss of plasmid derived 50 S formation. Compensatory mutations restoring helix 1 were assembled into functional 50 S subunits. We conclude that the helix 1 of 23 S rRNA is the main RNA determinant for ribosomal large-subunit assembly. Deletions in both the 5' and 3' strand of the processing stem reduced the ability of the 23 S rRNA to form ribosomal 50 S subunits. However, even the complete removal of either the 5' or the 3' strand of the processing stem did not abolish the 50 S assembly completely. Thus, processing stem facilitates, but is not essential for ass...Continue Reading

References

Oct 15, 1979·Journal of Molecular Biology·C C de Narvaez, H W Schaup
Feb 13, 1975·Nature·G MangiarottiF Altruda
Jun 25, 1992·Nucleic Acids Research·U Saarma, J Remme
Dec 20, 1992·Journal of Molecular Biology·R S Cormack, G A Mackie
Jan 1, 1990·Annual Review of Microbiology·A K Srivastava, D Schlessinger
Jun 1, 1991·Biochimie·K H Nierhaus
Jan 1, 1991·Annual Review of Biochemistry·H F Noller
Jan 1, 1988·Methods in Enzymology·C D SigmundE A Morgan
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel
Jan 1, 1984·Annual Review of Biochemistry·M NomuraG Baughman
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·T C KingD Schlessinger
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·V Nowotny, K H Nierhaus
Jan 1, 1994·Progress in Nucleic Acid Research and Molecular Biology·J M Zengel, L Lindahl
Dec 1, 1995·Microbiological Reviews·C CondonC L Squires
Nov 1, 1996·Journal of Molecular Biology·A LiivJ Remme

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Citations

Oct 16, 2012·Journal of Bacteriology·Nancy S Gutgsell, Chaitanya Jain
Jun 20, 2003·BMC Molecular Biology·Ular AllasJaanus Remme
Aug 2, 2005·BMC Molecular Biology·Aivar LiivJaanus Remme
Aug 15, 1998·Biological Chemistry·A LiivJ Remme
Jun 28, 2005·Molecular Microbiology·Elena Evguenieva-Hackenberg
Nov 24, 2021·RNA Biology·Elan A ShatoffKurt Fredrick

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