PMID: 9646949Jul 1, 1998Paper

Baseline diene conjugation in LDL lipids as a direct measure of in vivo LDL oxidation

Clinical Biochemistry
M AhotupaS Ylä-Herttuala

Abstract

To additionally test validity of the recently developed method (LDL baseline diene conjugation, LDL-BDC) for determination of circulating oxidized LDL. A detailed comparison between the ultracentrifugation and heparin precipitation methods for LDL isolation was performed to test suitability of the fast precipitation method. Validity of LDL-BDC as an indicator of circulating oxidized LDL was tested by comparing LDL-BDC to results obtained by the immunological autoantibody method. BDC values in LDL isolated by heparin precipitation did not differ from those isolated by sequential ultracentrifugation. While highest amount of diene conjugation was found in LDL (40% of that in serum), substantial amounts were also found in VLDL (31%) and HDL (25%). When analyzed in the same samples, assays for the titer of autoantibodies against oxidized LDL and LDL-BDC were found to show good correlation (r = 0.57, p = 0.001, n = 29). These results, together with thus far conducted studies on clinical applicability of the method, indicate that LDL-BDC is a promising candidate in search for a method for estimation of LDL oxidation in vivo.

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Citations

Oct 28, 2004·Lipids·Jukka-Pekka SuomelaHeikki Kallio
Dec 21, 2000·Current Atherosclerosis Reports·A L Catapano, E Tragni
Mar 19, 2004·Nuclear Medicine and Biology·G SobalH Sinzinger
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Jan 21, 2000·Free Radical Biology & Medicine·M Ahotupa, T J Asankari
Mar 3, 2007·Biological & Pharmaceutical Bulletin·Eunju ParkHyun-Dong Paik
Mar 15, 2003·Clinical Chemistry and Laboratory Medicine : CCLM·Patrizia BrizziSalvatore Musumeci
Apr 9, 2008·Journal of Clinical Biochemistry and Nutrition·Meral Baş Ozsoy, Aysun Pabuçcuoğlu
Jul 26, 2013·Journal of Nutritional Science and Vitaminology·Jungmi KimEunju Park
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Nov 10, 2009·Atherosclerosis·Jouni KarppiKristiina Nyyssönen
Oct 11, 2005·Clinical Biochemistry·Ozcan Erel
Jun 15, 2014·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Klára SzentmihályiAnna Blázovics
Jun 11, 1999·The American Journal of Cardiology·P KääpäM Ahotupa
Jun 21, 2015·Atherosclerosis·Petri KresanovOlli T Raitakari
Aug 20, 2010·Annals of Medicine·Markku AhotupaTommi Vasankari
Mar 5, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ravirajsinh N JadejaA V Ramachandran

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