Baseline expression profile of meiotic-specific genes in healthy fertile males

Fertility and Sterility
Carme NoguésCristina Templado

Abstract

To establish the quantitative gene-expression profile of nine meiotic genes involved in synapsis and chromosome cohesion (SYCP1, SPO11, MSH4, MSH5, MLH1, MLH3, PMS2, STAG3, and REC8) in healthy fertile males. Prospective study. Research university laboratory and clinical andrology service. Twenty healthy males of proven fertility underwent a vasectomy procedure and four infertile patients with Sertoli cell-only syndrome (SCOS). Analysis of testicular biopsies from 20 fertile males and four SCOS patients. Quantitative gene expression by real-time polymerase chain reaction in testicular biopsies. Four of the nine genes under study (PMS2, MLH3, MLH1, and REC8) are expressed in both fertile males and SCOS patients. The remaining five genes are only (SYCP1, SPO11, MSH4, and MSH5) or mainly (STAG3) expressed in fertile males, and thus they could be considered meiotic-specific genes. All genes analyzed are expressed at similar levels among fertile individuals Gene expression levels reported in this study could be considered the gene expression profile of fertile population, and could be used to compare with the expression pattern of infertile patients. Expression of meiotic-specific genes could be used as a clinical diagnosis tool to ...Continue Reading

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Citations

Mar 7, 2014·The New England Journal of Medicine·Sandrine CaburetEric Vilain
Oct 8, 2009·Clinica Chimica Acta; International Journal of Clinical Chemistry·Keqian XuYaoyun Xiang
Dec 6, 2019·Frontiers in Genetics·Wen-Juan XiaoJuan Du
Oct 14, 2010·Current Opinion in Urology

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