Basement membrane ligands initiate distinct signalling networks to direct cell shape.

Matrix Biology : Journal of the International Society for Matrix Biology
Michael J RandlesRachel Lennon

Abstract

Cells have evolved mechanisms to sense the composition of their adhesive microenvironment. Although much is known about general mechanisms employed by adhesion receptors to relay signals between the extracellular environment and the cytoskeleton, the nuances of ligand-specific signalling remain undefined. Here, we investigated how glomerular podocytes, and four other basement membrane-associated cell types, respond morphologically to different basement membrane ligands. We defined the composition of the respective adhesion complexes using mass spectrometry-based proteomics. On type IV collagen, all epithelial cell types adopted a round morphology, with a single lamellipodium and large adhesion complexes rich in actin-binding proteins. On laminin (511 or 521), all cell types attached to a similar degree but were polygonal in shape with small adhesion complexes enriched in endocytic and microtubule-binding proteins. Consistent with their distinctive morphologies, cells on type IV collagen exhibited high Rac1 activity, while those on laminin had elevated PKCα. Perturbation of PKCα was able to interchange morphology consistent with a key role for this pathway in matrix ligand-specific signalling. Therefore, this study defines the s...Continue Reading

Citations

Aug 26, 2020·Nature Reviews. Nephrology·Richard W NaylorRachel Lennon
Jan 19, 2021·Wellcome Open Research·Salman B HosawiRachel Lennon
Jun 7, 2020·Matrix Biology : Journal of the International Society for Matrix Biology·Kristine Y DeLeon-PennellMerry L Lindsey
Jul 6, 2020·Matrix Biology : Journal of the International Society for Matrix Biology·Anna MichopoulouPatricia Rousselle
Sep 21, 2020·Progress in Retinal and Eye Research·Sayon Roy, Dongjoon Kim
Jun 2, 2021·Matrix Biology : Journal of the International Society for Matrix Biology·Stefano BarberaMaurizio Orlandini
Jul 2, 2021·Matrix Biology Plus·Jared A Mereness, Thomas J Mariani
May 30, 2021·Journal of the American Society of Nephrology : JASN·Michael RandlesRachel Lennon
Sep 13, 2021·Biophysical Journal·Kerbaï Saïd ErouméAurélie Carlier

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Datasets Mentioned

BETA
PXD017913

Methods Mentioned

BETA
flow cytometry
pulldown
pull down
fluorescence imaging
PMA
GTPase
FCS
fluorescence-activated cell sorting
affinity purification

Software Mentioned

Protein Interaction Network Analysis
Fiji imageJ
Excel
T4MeV
Progenesis
Mascot
Proteome
Mascot Daemon
Scaffold
Cytoscape

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