Basic and Clinical Evidence of an Alternative Method to Produce Vivo Nanofat

Chinese Medical Journal
Hong-Sen BiE Xiao

Abstract

Fat grafting technologies are popularly used in plastic and reconstructive surgery. Due to its size limitation, it is hard to directly inject untreated fat tissue into the dermal layer. Nanofat, which was introduced by Tonnard, solves this problem by mechanically emulsifying fat tissue. However, the viability of the cells was greatly destroyed. In this study, we reported a new method by "gently" digesting the fat tissue to produce viable adipocytes, progenitors, and stromal stem cells using collagenase I digestion and centrifugation. This was named "Vivo nanofat". Human liposuction aspirates were obtained from five healthy female donors with mean age of 28.7 ± 5.6 years. Colony-forming assay, flow cytometry analysis, and adipogenic and osteogenic induction of the adherent cells from the Vivo nanofat were used to characterize the adipose mesenchymal stem cells (MSCs). To investigate in vivo survival, we respectively injected Vivo nanofat and nanofat subcutaneously to the back of 8-week-old male BALB/c nude mice. Samples were harvested 2 days, 2 weeks, and 4 weeks postinjection for measurement, hematoxylin and eosin staining, and immunostaining. Our results showed that the Vivo nanofat contained a large number of colony-forming c...Continue Reading

References

Jan 21, 2012·Plastic and Reconstructive Surgery·Hitomi EtoKotaro Yoshimura
Jun 30, 2012·Plastic and Reconstructive Surgery·Phanette GirRod J Rohrich
Jun 21, 2013·Plastic and Reconstructive Surgery·Patrick TonnardHeidi Declercq
Sep 15, 2016·Plastic and Reconstructive Surgery·Takanobu MashikoKotaro Yoshimura
Jun 8, 2017·Plastic and Reconstructive Surgery·Nicolas BertheuilBenoit Chaput

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Methods Mentioned

BETA
flow cytometry

Software Mentioned

GraphPad
GraphPad Prism

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