Jan 1, 1976

Basic and neutral amino acid transport in Aspergillus nidulans

Journal of General Microbiology
M PiotrowskaE Zakrzewska

Abstract

Arginine and methionine transport by Aspergillus nidulans mycelium was investigated. A single uptake system is responsible for the transport of arginine, lysine and ornithine. Transport is energy-dependent and specific for these basic amino acids. The Km value for arginine is 1 X 10(-5) M, and Vmax is 2-8 nmol/mg dry wt/min; Km for lysine is 8 X 10(-6) M; Kt for lysine as inhibitor of arginine uptake is 12 muM, and Ki for ornithine is mM. On minimal medium, methionine is transported with a Km of 0-I mM and Vmax about I nmol/mg dry wt/min; transport is inhibited by azide. Neutral amnio acids such as serine, phenylalanine and leucine are probably transported by the same system, as indicated by their inhibition of methionine uptake and the existence of a mutant specifically impaired in their transport. The recessive mutant nap3, unable to transport neutral amino acids, was isolated as resistant to selenomethionine and p-fluorophenylanine. This mutant has unchanged transport of methionine by general and specific sulphur-regulated permeases.

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Mentioned in this Paper

Arginine hydrochloride
Histidine
Aspergillus Niger Infection
Methionine Transport
L-Selenomethionine
Amino Acids, I.V. solution additive
Uptake
Ornithine Monoacetate, (L)-Isomer
Sulfur
Proline

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