Basic apoptotic mechanisms of lead toxicity in human leukemia (HL-60) cells.

International Journal of Environmental Research and Public Health
Clement G YedjouPaul B Tchounwou

Abstract

Lead exposure represents a medical and public health emergency, especially in children consuming high amounts of lead-contaminated flake paints. It may also cause hematological effects to people of all ages. Recent studies in our laboratory have indicated that apoptosis may be associated with the lead-induced oxidative stress and DNA damage. However, the mechanisms underlying its effect on lymphocytes are still largely unknown. Therefore, the aim of the present study was to investigate the apoptotic mechanisms of lead nitrate [Pb(NO(3))(2)] using HL-60 cells as a test model. HL-60 cells were treated with different concentrations of Pb(NO(3))(2) for 24 h prior to cell viability assay and flow cytometry assessment. The results obtained from the trypan blue exclusion test indicated that at very low concentration, Pb(NO(3))(2) has no effect on the viability of HL-60 cells. A significant (p < 0.05) decrease in cell viability was observed when exposed to high level of Pb(NO(3))(2). Data generated from the flow cytometric assessment indicated that Pb(NO(3))(2) exposure significantly (p < 0.05) increased the proportion of annexin V positive cells (apoptotic cells) compared to the control. Pb(NO(3))(2) induced apoptosis of HL-60 cells w...Continue Reading

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Citations

Dec 31, 2010·Applied Microbiology and Biotechnology·Jurrian Vanden Bussche, Eduardo V Soares
Aug 2, 2012·International Journal of Environmental Research and Public Health·Yuelin YangPin Sun
Jul 16, 2014·Apoptosis : an International Journal on Programmed Cell Death·Gaspar Banfalvi
Dec 26, 2015·International Journal of Environmental Research and Public Health·Clement G YedjouPaul B Tchounwou
Oct 13, 2011·Drug and Chemical Toxicology·Kilarkaje Narayana, Raj Raghupathy
Aug 27, 2011·Journal of Hazardous Materials·Changming Dou, Jie Zhang
Jan 11, 2011·Bioorganic & Medicinal Chemistry·Francisco W A BarrosClaudia do Ó Pessoa
May 9, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Gaspar BanfalviGabor Nagy
Jun 21, 2018·The Journal of Biological Chemistry·David A HannaAmit R Reddi
Jan 25, 2017·Applied and Environmental Microbiology·Pallavee Srivastava, Meenal Kowshik
Sep 30, 2019·Journal of Epidemiology and Community Health·Yukari YamadaShingo Fukuma
Oct 18, 2014·Journal of Perinatal Medicine·Sabrina L O'DwyerJohn Anthony
Jun 20, 2020·Biological Trace Element Research·Emilia MetrykaIrena Baranowska-Bosiacka
Apr 26, 2018·BioMed Research International·Shin Hye KimJae-Jun Song
May 24, 2019·Human & Experimental Toxicology·M S ManiH S Dsouza

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Methods Mentioned

BETA
Assay
flow cytometry

Software Mentioned

CellQuest

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis