PMID: 9536826Apr 16, 1998Paper

Basic calcium phosphate crystals induce synthesis and secretion of 92 kDa gelatinase (gelatinase B/matrix metalloprotease 9) in human fibroblasts

Annals of the Rheumatic Diseases
G M McCarthyT Pourmotabbed

Abstract

Synovial fluid basic calcium phosphate (BCP) crystals are associated with severe joint degeneration accompanied by synovial hypertrophy. The metalloprotease 92 kDa gelatinase (MMP-9) has been implicated in the degradation of extracellular matrix in osteoarthritis, but the ability of BCP crystals to induce gelatinase in human fibroblasts or in adult porcine chondrocytes has not previously been studied. The hypothesis that the mitogenic response to BCP crystals is accompanied by induction and secretion of MMP-9 was studied. MMP-9 messenger RNA (mRNA) was detected by northern blot and reverse transcription-polymerase chain reaction (RT-PCR). Gelatinase secretion was identified by western blot and zymography of conditioned media. BCP crystals caused a concentration dependent induction of MMP-9 mRNA accumulation and protein secretion in human fibroblasts but not in adult porcine chondrocytes. BCP crystals induce MMP-9 production by HF but not adult porcine chondrocytes. Fibroblast MMP-9 may be an important mediator of the joint destruction associated with synovial fluid BCP crystals.

Citations

Mar 22, 2002·Progress in Retinal and Eye Research·Jeremy M Sivak, M Elizabeth Fini
Apr 23, 2004·Current Opinion in Rheumatology·Finbar D O'Shea, Geraldine M McCarthy
Apr 20, 2005·Current Opinion in Rheumatology·Herman S Cheung
Dec 21, 2000·Current Rheumatology Reports·H S Cheung
Dec 21, 2000·Current Rheumatology Reports·N Olmez, H R Schumacher
Dec 21, 2000·Current Rheumatology Reports·G M McCarthy
Apr 8, 2014·Rheumatic Diseases Clinics of North America·Ann K Rosenthal, Lawrence M Ryan
Oct 3, 1999·The Journal of Bone and Joint Surgery. American Volume·K L JensenC A Rockwood
Jun 5, 1999·Rheumatic Diseases Clinics of North America·L M Ryan, H S Cheung

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