Basic fibroblast growth factor affects neuronal migration and differentiation in normotypic cell cultures from the cochleovestibular ganglion of the chick embryo

Experimental Neurology
W A HossainD K Morest

Abstract

To study the role of basic fibroblast growth factor (FGF-2) in the development of sensory neurons, the cochleovestibular ganglion of the chicken embryo provides a well-characterized structure. This permits use of morphological markers in a cell culture preparation comparable to the normal embryo (normocytic). Otocysts were explanted from white leghorn embryos at Hamburger-Hamilton Stages 14-16, when ganglion cell precursors normally start migrating from the otic epithelium. The cultures were supplemented with either fetal bovine serum or human recombinant FGF-2 (in defined medium or serum) for 2 or 5 days. FGF-2 increased explant growth, neuroblast migration, and neurite outgrowth 2- to 10-fold in the first 2 days. Neuronal morphology appeared within 2-3 days with FGF-2 but required at least 4-5 days with serum. FGF-2 in defined medium stimulated early migration and differentiation, but without serum led to degeneration after 5 days. In serum, growth was later and slower but continued for at least 3 weeks. When explants were cultured in serum with a neutralizing antibody to FGF-2, but no FGF added, neuroblast migration and elongation were decreased by 2- to 4-fold, compared to serum alone. Immunocytochemistry demonstrated FGF r...Continue Reading

Citations

Jul 18, 2009·Journal of Neuroscience Research·J FengD K Morest
Jun 20, 1998·Mechanisms of Development·M Torres, F Giráldez
Dec 12, 2012·Developmental Biology·Androulla EconomouMark Maconochie
Feb 21, 2004·Developmental Biology·Berta AlsinaFernando Giraldez
Feb 11, 2014·Developmental Biology·Esther C MaierTanya T Whitfield
Dec 3, 1999·Journal of Neuroscience Research·R HendriksL K Kaczmarek
May 2, 2001·Journal of Neurochemistry·E CarniceroT Schimmang
Apr 9, 2021·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Bruce B Riley
Nov 19, 1997·Progress in Neurobiology·A F Rocha

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