Basic fibroblast growth factor destabilizes osteonectin mRNA in osteoblasts

The American Journal of Physiology
Anne M Delany, E Canalis

Abstract

Osteonectin (secreted protein acidic and rich in cysteine, 40-kDa basement membrane) is a glycoprotein abundantly expressed in bone and in other tissues undergoing active remodeling. Fibroblast growth factors (FGFs) are important in skeletal development and fracture repair, events associated with extracellular matrix remodeling. We used the murine osteoblastic cell line MC3T3 to determine whether basic FGF (bFGF) regulates osteonectin expression in bone. Northern blot analysis showed that bFGF decreased osteonectin transcripts in a dose- and time-dependent manner. This regulation was independent of the mitogenic effect of bFGF but was dependent on new protein synthesis. Immunoprecipitation of [35S]methionine-cysteine osteoblast-conditioned medium and cell layer proteins showed that bFGF decreased osteonectin synthesis. Nuclear runoff assays failed to reveal regulation of osteonectin gene transcription by bFGF. However, bFGF dramatically decreased the stability of osteonectin mRNA in transcriptionally arrested osteoblasts. This destabilization of osteonectin mRNA may be one means by which bFGF regulates extracellular matrix remodeling.

References

Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·E W RainesE H Sage
Feb 25, 1992·Nucleic Acids Research·I Oberbäumer
Aug 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·N S FedarkoU Vetter
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S E Funk, E H Sage
Jan 1, 1990·Connective Tissue Research·M F YoungJ D Termine
Mar 1, 1989·Calcified Tissue International·Y DoiH Shimokawa
Aug 1, 1988·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T L McCarthyE Canalis
May 1, 1988·DNA·P E DanielsonJ G Sutcliffe
Jul 5, 1983·Journal of Molecular Biology·R ZandomeniR Weinmann
May 1, 1994·The Journal of Cell Biology·T F LaneE H Sage
Aug 1, 1995·Current Opinion in Genetics & Development·T P Yamaguchi, J Rossant
Sep 14, 1995·Biochemical and Biophysical Research Communications·M M HurleyM S Hibbs
Feb 1, 1993·The Journal of Experimental Zoology·L PurcellM Ringuette

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Citations

Oct 29, 2009·Journal of Cell Communication and Signaling·Anne M Delany, Kurt David Hankenson
Jun 20, 1998·The Journal of Biological Chemistry·A M Delany, E Canalis
Mar 28, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Adele J VincentA Jane Roskams
Sep 28, 2007·Pflügers Archiv : European journal of physiology·Oleg E Osadchii
Aug 18, 2009·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Mansoureh TabatabaeifarUNKNOWN ESCAPE Trial Group
Sep 30, 2014·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Neha S DoleAnne M Delany
Dec 8, 2004·The Journal of Biological Chemistry·Stephen D WaterfordFred S Gorelick
Oct 20, 2000·Pancreas·E Niebergall-RothM V Singer
Nov 16, 2010·Phytotherapy Research : PTR·Hyun-Sun LeeHyung Joo Suh
Jul 22, 2014·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Hendrik MommaertsPrzemko Tylzanowski
Jan 7, 2000·Journal of Cellular Physiology·R C PereiraE Canalis
Mar 15, 2005·The Journal of Pharmacology and Experimental Therapeutics·Dinesh GautamJürgen Wess
Oct 25, 2006·Annual Review of Physiology·Ashok K SalujaVikas Dudeja
Dec 18, 2007·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·A M DelanyE S Kurland
Oct 7, 2006·Current Opinion in Gastroenterology·M A Shetzline, R A Liddle
Apr 5, 2000·The Journal of Clinical Investigation·A M DelanyE Canalis
Mar 31, 2021·Molecular and Cellular Endocrinology·Pedro CarriereClaudia Gentili
Feb 27, 2001·Matrix Biology : Journal of the International Society for Matrix Biology·R A Brekken, E H Sage
Dec 5, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·R A Brekken, E H Sage
Jun 11, 2002·Biochemical and Biophysical Research Communications·Shinji SakanoHisao Seo
Nov 22, 2005·Biochemical and Biophysical Research Communications·T NaganawaM M Hurley
Dec 5, 2009·Seminars in Cell & Developmental Biology·Alexandre Chlenski, Susan L Cohn

❮ Previous
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