Basic fibroblast growth factor expression in isolated small human ovarian follicles

Molecular Human Reproduction
J H QuennellP R Hurst

Abstract

Basic fibroblast growth factor (bFGF) is involved in cell proliferation, differentiation, and angiogenesis. It has long been known that bFGF acts as a powerful mitogen for various mammalian granulosa cells in culture. To investigate the possible involvement of bFGF expression in follicle initiation and growth in vivo, we performed nested RT-PCR on ovarian cortical biopsies and quantitative PCR on human follicle populations isolated by laser capture microdissection. Using morphological criteria, follicles were characterized as putative non-growing, primary, or small secondary. RNA was extracted from samples, reverse-transcribed, and relative gene expression levels determined with TaqMan real-time PCR, using 18S rRNA as the endogenous control. Results confirmed bFGF expression in human adult ovarian cortex, and in the isolated follicles a down-regulation of bFGF mRNA was evident as small follicles develop. This study demonstrates a possible relationship between bFGF mRNA expression and follicle development.

Citations

Jul 1, 2010·Zygote : the Biology of Gametes and Early Embryos·Isana M A FrotaJosé R V Silva
Feb 18, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·C L LuJ Qiao

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