Basic fibroblast growth factor prolongs the proliferation of rat cortical progenitor cells in vitro without altering their cell cycle parameters

Cerebral Cortex
J F CavanaghJ G Parnavelas

Abstract

Basic fibroblast growth factor (bFGF) has been shown to influence the survival, proliferation and differentiation of a variety of cell types in the nervous system. In this investigation we have examined the action of bFGF on: (i) the rate of proliferation; (ii) cell cycle parameters; (iii) the maintenance of cell division; (iv) the recruitment of quiescent cells; and (v) the degree of differentiation of cortical progenitor cells in cultures prepared from E16 rat embryos. The proliferation rate (labelling index) of cortical progenitor cells doubled in the presence of bFGF over 48 h. However, the lengths of the cell cycle phases were unchanged. Clones marked with a recombinant retrovirus on the first day in vitro (DIV) grew significantly larger in the presence of bFGF. Furthermore, many of the clones examined in control cultures had ceased to divide after a maximum of four cell cycles, whereas almost all clonally related cells were still dividing in the presence of bFGF 4 days later, i.e. for at least six cell cycles. Basic FGF also stimulated the division of quiescent progenitor cells, which otherwise would have differentiated or undergone cell death. The degree of neuronal and glial differentiation was studied after 5 DIV using...Continue Reading

Citations

Sep 4, 2007·Cellular and Molecular Neurobiology·Sheng YeYan-jun Zeng
Apr 12, 2007·BMC Developmental Biology·James PritchettBagirathy Nadarajah
Jan 27, 2009·PLoS Biology·Arthur D LanderAnne L Calof
Apr 22, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Luis R Hernández-MirandaWilliam D Andrews
Apr 18, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Mason L YehWilliam D Andrews
Feb 16, 2005·Developmental Neuroscience·Kelvin C Luk, Abbas F Sadikot
May 16, 2015·The Journal of Comparative Neurology·William D AndrewsJohn G Parnavelas
Sep 30, 2005·Journal of Neurochemistry·Julie A Siegenthaler, Michael W Miller
Jun 8, 2007·Journal of Anatomy·William D AndrewsJohn G Parnavelas
Apr 25, 2006·Developmental Biology·Mahmud Bani-YaghoubMarianna Sikorska
Jun 2, 2009·Journal of Pharmacological Sciences·Noritaka NakamichiYukio Yoneda
Jun 29, 2010·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Sabrina ZechelOliver von Bohlen und Halbach
Apr 8, 2017·Brain Structure & Function·Abigail C KillenWilliam D Andrews
Mar 11, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Rosanna Dono
Nov 20, 2016·Brain Structure & Function·William D AndrewsJohn G Parnavelas
Oct 14, 2018·Brain Structure & Function·Fani MemiWilliam D Andrews

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