Basic fibroblast growth factor reduces scar by inhibiting the differentiation of epidermal stem cells to myofibroblasts via the Notch1/Jagged1 pathway

Stem Cell Research & Therapy
Peng WangJu-Lin Xie

Abstract

Basic fibroblast growth factor (bFGF) plays an important role in promoting wound healing and reducing scar, but the possible molecular mechanisms are still unclear. Our previous studies have found that activating the Notch1/Jagged1 pathway can inhibit the differentiation of epidermal stem cells (ESCs) to myofibroblasts (MFB). Herein, we document that bFGF reduces scar by inhibiting the differentiation of ESCs to MFB via activating the Notch1/Jagged1 pathway. In in-vitro study, ESCs were isolated from 10 neonatal SD rats (1-3 days old), cultured in keratinocyte serum-free medium, and divided into six groups: bFGF group, bFGF + SU5402 group, bFGF + DAPT group, siJagged1 group, bFGF + siJagged1 group, and control group. Jagged1 of the ESCs in the siJagged1 group and bFGF + siJagged1 group was knocked down by small-interfering RNA transfection. Expression of ESC markers (CK15/CK10), MFB markers (α-SMA, Collagen I, Collagen III), and Notch1/Jagged1 components (Jagged1, Notch1, Hes1) was detected by FCM, qRT-PCR, and western blot analysis to study the relationships of bFGF, ESCs, and Notch1/Jagged1 pathway. In in-vivo study, the wound healing time and scar hyperplasia were observed on rabbit ear scar models. The quality of wound heal...Continue Reading

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Citations

Aug 1, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Zhen-Hua GaoYun Zhang
Nov 22, 2018·Phytotherapy Research : PTR·Ronghua YangJulin Xie
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Mar 4, 2021·Liver International : Official Journal of the International Association for the Study of the Liver·Tatjana Seitz, Claus Hellerbrand
May 6, 2021·Biomolecules·Meera KrishnanPrasad Abnave
May 29, 2021·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·Na LiDahai Hu

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Methods Mentioned

BETA
transfection
PCR
electrophoresis
light microscopy
flow cytometry

Software Mentioned

SPSS
kern2em
PRISM5
Image Pro - Plus
Graph Prism
ImageJ

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