Basic fibroblast growth factor regulates IGF-I binding proteins in the clonal osteoblastic cell line MC3T3-E1

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
M M HurleyY Hakeda

Abstract

In previous studies, we reported that basic fibroblast growth factor (bFGF) regulates insulin-like growth factor messenger RNAs and protein levels in the osteoblastic MC3T3-E1 cells. In the present study, we examined the expression of insulin-like growth factor binding proteins (IGFBPs) in MC3T3-E1 cells and determined whether bFGF altered IGFBP mRNAs and protein levels. Since previous studies suggested that IGFBPs can inhibit DNA synthesis stimulated by IGF-I, we wondered whether the mitogenic effect of bFGF was altered by exogenous IGFBP-3. Confluent MC3T3-E1 cells were serum-deprived for 24 h and then treated with bFGF for 6-24 h. In control cultures, MC3T3-E1 cells expressed the mRNAs for IGF-I, IGF-II, and IGFBP-2, 4, 5, and 6 but not IGFBP-1 or 3. A 24 h treatment with bFGF at 10(-8) M decreased IGF-I mRNA by 97%, IGF-II mRNA by 73%, IGFBP-2 by 64%, IGFBP-4 by 73%, IGFBP-5 by 95%, and IGFBP-6 by 65%. The inhibitory effect of bFGF on IGF-I and IGFBP mRNA levels was not altered by aphidicolin, an inhibitor of cell replication. bFGF 10 nM decreased IGF-I levels determined by radioimmunoassay after acidification by 45% and 72% at 24 and 48 h, respectively. Western ligand blot for IGF binding proteins revealed that MC3T3-E1 ce...Continue Reading

References

Jun 1, 1975·Archives of Biochemistry and Biophysics·J ZapfE R Froesch
Nov 1, 1977·The Journal of Clinical Endocrinology and Metabolism·R L Hintz, F Liu
Dec 1, 1991·Journal of Cellular Physiology·J VillaudyL Harel
Nov 1, 1991·Endocrinology·E CanalisT L McCarthy
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S MohanD J Baylink
Jan 1, 1989·Progress in Growth Factor Research·R C Baxter, J L Martin
Apr 28, 1989·Biochemical and Biophysical Research Communications·C SchmidE R Froesch
Feb 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S AmarnaniS Mohan
Feb 1, 1993·The Journal of Bone and Joint Surgery. American Volume·S B TrippelS R Doctrow

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Citations

Feb 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J L SolanR A Terkeltaub
Nov 16, 2013·Cellular and Molecular Life Sciences : CMLS·H Al-KharobiJ Beattie
May 20, 2014·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Gang XiDavid R Clemmons
Aug 1, 2015·Endocrinology·Victoria E DeMambroClifford J Rosen
Oct 5, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S S KelpkeJ A Thompson
May 12, 1998·The American Journal of Physiology·R N HanA K Tanswell
Aug 1, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M Shiokawa-SawadaY Hakeda
Sep 18, 2004·Journal of Biomedical Materials Research. Part a·S S KelpkeJ A Thompson

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