Basic principles and technologies for deciphering the genetic map of cancer.

World Journal of Surgery
Georgios VoidonikolasMarie-Claude Gingras

Abstract

The progress achieved in the field of genomics in recent years is leading medicine to adopt a personalized model in which the knowledge of individual DNA alterations will allow a targeted approach to cancer. Using pancreatic cancer as a model, we discuss herein the fundamentals that need to be considered for the high throughput and global identification of mutations. These include patient-related issues, sample collection, DNA isolation, gene selection, primer design, and sequencing techniques. We also describe the possible applications of the discovery of DNA changes to the approach of this disease and cite preliminary efforts where the knowledge has been translated into the clinical or preclinical setting.

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Citations

Mar 23, 2011·World Journal of Surgery·F Charles BrunicardiChangyi Chen
Jan 13, 2011·World Journal of Surgery·Theresa R HarringJohn A Goss
Feb 3, 2011·World Journal of Surgery·Courtney J BalentineF Charles Brunicardi
Oct 4, 2014·Journal of Hematology & Oncology·Alan D SmithTimothy A Yap
Apr 3, 2014·International Journal of Nursing Practice·Hamid Sharif NiaNasim Bahrami
Jan 25, 2012·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Ben TranLillian L Siu
Jan 1, 2011·Ophthalmology and Eye Diseases·Bp BieloryRe Turbin
Dec 15, 2015·World Journal of Surgery·Krishan JosephDavid A Watters
Mar 29, 2014·Biomechanics and Modeling in Mechanobiology·Alkiviadis TsamisDavid A Vorp

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